DDAH2
Putative hydrolase DDAH2
Also known as: DDAH2_HUMAN, G6A
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O95865
- Gene
- DDAH2
- Ensembl
- ENSG00000213722
- Chromosome
- 6
- Canonical length
- 285 aa
- Protein class
- Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
- Subcellular location
- Mitochondria
OverviewNCBI Gene
This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014]
Canonical amino-acid sequenceUniProt
285 residues, UniProt reviewed canonical sequence.
>O95865|DDAH2
1 MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG KLRQRLGLQL
61 LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV DGVRKALQDL GLRIVEIGDE
121 NATLDGTDVL FTGREFFVGL SKWTNHRGAE IVADTFRDFA VSTVPVSGPS HLRGLCGMGG
181 PRTVVAGSSD AAQKAVRAMA VLTDHPYASL TLPDDAAADC LFLRPGLPGV PPFLLHRGGG
241 DLPNSQEALQ KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against DDAH2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.25
- Highest tissue expression
- 125 nTPM
Expression across tissuesHPA
Tissue
- cervix: 125 nTPM
- blood vessel: 109 nTPM
- adipose tissue: 105 nTPM
- endometrium: 102 nTPM
- vagina: 99 nTPM
- fallopian tube: 93 nTPM
Single-cell type
- decidual stromal cells: 130 nCPM
- colonocytes: 101 nCPM
- breast secretory cells: 101 nCPM
- hofbauer cells: 95 nCPM
- kupffer cells: 89 nCPM
- peritubular myoid cells: 83 nCPM
Immune cell
- basophil: 142 nTPM
- eosinophil: 135 nTPM
- plasmacytoid DC: 94 nTPM
- memory B-cell: 50 nTPM
- non-classical monocyte: 45 nTPM
- naive B-cell: 40 nTPM
Brain region
- hypothalamus: 42 nTPM
- medulla oblongata: 38 nTPM
- spinal cord: 38 nTPM
- white matter: 34 nTPM
- midbrain: 30 nTPM
- thalamus: 28 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.86
- gnomAD pLI
- 0.01
- gnomAD missense Z
- 2.31
- DepMap mean gene effect
- 0.02
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of DDAH2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads DDAH2 as an antibody target. Whether an autoantibody or antibody against DDAH2 could matter depends on whether native DDAH2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
DDAH2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label DDAH2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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