CYP2F1

Protein identityUniProt · HPA

UniProt accession

P24903

Gene

CYP2F1

Ensembl

ENSG00000197446

Chromosome

19

Canonical length

491 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Secretome location

Intracellular and membrane

OverviewNCBI Gene

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

491 residues, UniProt reviewed canonical sequence.

>P24903|CYP2F1
     1  MDSISTAILL LLLALVCLLL TLSSRDKGKL PPGPRPLSIL GNLLLLCSQD MLTSLTKLSK
    61  EYGSMYTVHL GPRRVVVLSG YQAVKEALVD QGEEFSGRGD YPAFFNFTKG NGIAFSSGDR
   121  WKVLRQFSIQ ILRNFGMGKR SIEERILEEG SFLLAELRKT EGEPFDPTFV LSRSVSNIIC
   181  SVLFGSRFDY DDERLLTIIR LINDNFQIMS SPWGELYDIF PSLLDWVPGP HQRIFQNFKC
   241  LRDLIAHSVH DHQASLDPRS PRDFIQCFLT KMAEEKEDPL SHFHMDTLLM TTHNLLFGGT
   301  KTVSTTLHHA FLALMKYPKV QARVQEEIDL VVGRARLPAL KDRAAMPYTD AVIHEVQRFA
   361  DIIPMNLPHR VTRDTAFRGF LIPKGTDVIT LLNTVHYDPS QFLTPQEFNP EHFLDANQSF
   421  KKSPAFMPFS AGRRLCLGES LARMELFLYL TAILQSFSLQ PLGAPEDIDL TPLSSGLGNL
   481  PRPFQLCLRP R

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYP2F1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

1.8 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.24

gnomAD pLI

0

gnomAD missense Z

0

DepMap mean gene effect

-0.01

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• epoxygenase P450 pathway
• response to toxic substance
• xenobiotic metabolic process
• naphthalene catabolic process

Molecular functions

• arachidonate epoxygenase activity
• heme binding
• iron ion binding
• monooxygenase activity
• oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
• oxygen binding

Cellular components

• cytoplasm
• endoplasmic reticulum membrane
• intracellular membrane-bounded organelle

Protein domainsUniProt · Pfam · InterPro

• Cytochrome P450
• Cytochrome P450, E-class, group I
• Cytochrome P450, conserved site
• Cytochrome P450 superfamily
• Cytochrome P450 family 2
• Cytochrome P450
• Cytochrome P450, CYP2 family

KeywordsUniProt

• Endoplasmic reticulum
• Heme
• Iron
• Membrane
• Metal-binding
• Microsome
• Monooxygenase
• Oxidoreductase