CYP2E1

Protein identityUniProt · HPA

UniProt accession

P05181

Gene

CYP2E1

Ensembl

ENSG00000130649

Chromosome

10

Canonical length

493 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Endoplasmic reticulum,Mitochondria

OverviewNCBI Gene

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

493 residues, UniProt reviewed canonical sequence.

>P05181|CYP2E1
     1  MSALGVTVAL LVWAAFLLLV SMWRQVHSSW NLPPGPFPLP IIGNLFQLEL KNIPKSFTRL
    61  AQRFGPVFTL YVGSQRMVVM HGYKAVKEAL LDYKDEFSGR GDLPAFHAHR DRGIIFNNGP
   121  TWKDIRRFSL TTLRNYGMGK QGNESRIQRE AHFLLEALRK TQGQPFDPTF LIGCAPCNVI
   181  ADILFRKHFD YNDEKFLRLM YLFNENFHLL STPWLQLYNN FPSFLHYLPG SHRKVIKNVA
   241  EVKEYVSERV KEHHQSLDPN CPRDLTDCLL VEMEKEKHSA ERLYTMDGIT VTVADLFFAG
   301  TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRIPA IKDRQEMPYM DAVVHEIQRF
   361  ITLVPSNLPH EATRDTIFRG YLIPKGTVVV PTLDSVLYDN QEFPDPEKFK PEHFLNENGK
   421  FKYSDYFKPF STGKRVCAGE GLARMELFLL LCAILQHFNL KPLVDPKDID LSPIHIGFGC
   481  IPPRYKLCVI PRS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYP2E1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

9,697 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CYP2E1.

Disease | ImmuneIEDB

Conditions an epitope on CYP2E1 was assayed in.

• drug-induced hepatitis B cell
• hepatitis C B cell

Disease | AutoantibodyPubMed

Conditions in which antibodies against CYP2E1 are reported. Each links to that disease's full target list.

• Hepatitis C, Chronic 3

Showing 1 of 2 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for CYP2E1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.17

gnomAD pLI

0

gnomAD missense Z

0.88

DepMap mean gene effect

0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• epoxygenase P450 pathway
• lipid hydroxylation
• long-chain fatty acid biosynthetic process
• long-chain fatty acid metabolic process
• monoterpenoid metabolic process
• response to bacterium
• steroid metabolic process
• xenobiotic metabolic process
• 4-nitrophenol metabolic process
• benzene metabolic process
• carbon tetrachloride metabolic process
• halogenated hydrocarbon metabolic process

Molecular functions

• arachidonate epoxygenase activity
• enzyme binding
• heme binding
• Hsp70 protein binding
• Hsp90 protein binding
• iron ion binding
• long-chain fatty acid omega-1 hydroxylase activity
• monooxygenase activity
• oxidoreductase activity
• oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
• oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
• oxygen binding
• 4-nitrophenol 2-monooxygenase activity

Cellular components

• cytoplasm
• endoplasmic reticulum membrane
• mitochondrial inner membrane

Protein domainsUniProt · Pfam · InterPro

• Cytochrome P450
• Cytochrome P450, E-class, group I
• Cytochrome P450, conserved site
• Cytochrome P450 superfamily
• Cytochrome P450 family 2
• Cytochrome P450
• Cytochrome P450, E-class, group I, CYP2E-like

KeywordsUniProt

• Endoplasmic reticulum
• Fatty acid metabolism
• Heme
• Iron
• Lipid metabolism
• Membrane
• Metal-binding
• Microsome
• Mitochondrion
• Mitochondrion inner membrane
• Monooxygenase
• NADP
• Oxidoreductase

InteractionsUniProt · HPA

Protein binding partners of CYP2E1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HSP90