CYP1A2

Protein identityUniProt · HPA

UniProt accession

P05177

Gene

CYP1A2

Ensembl

ENSG00000140505

Chromosome

15

Canonical length

516 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Predicted membrane proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

516 residues, UniProt reviewed canonical sequence.

>P05177|CYP1A2
     1  MALSQSVPFS ATELLLASAI FCLVFWVLKG LRPRVPKGLK SPPEPWGWPL LGHVLTLGKN
    61  PHLALSRMSQ RYGDVLQIRI GSTPVLVLSR LDTIRQALVR QGDDFKGRPD LYTSTLITDG
   121  QSLTFSTDSG PVWAARRRLA QNALNTFSIA SDPASSSSCY LEEHVSKEAK ALISRLQELM
   181  AGPGHFDPYN QVVVSVANVI GAMCFGQHFP ESSDEMLSLV KNTHEFVETA SSGNPLDFFP
   241  ILRYLPNPAL QRFKAFNQRF LWFLQKTVQE HYQDFDKNSV RDITGALFKH SKKGPRASGN
   301  LIPQEKIVNL VNDIFGAGFD TVTTAISWSL MYLVTKPEIQ RKIQKELDTV IGRERRPRLS
   361  DRPQLPYLEA FILETFRHSS FLPFTIPHST TRDTTLNGFY IPKKCCVFVN QWQVNHDPEL
   421  WEDPSEFRPE RFLTADGTAI NKPLSEKMML FGMGKRRCIG EVLAKWEIFL FLAILLQQLE
   481  FSVPPGVKVD LTPIYGLTMK HARCEHVQAR LRFSIN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CYP1A2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

478 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for CYP1A2 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.34

gnomAD pLI

0

gnomAD missense Z

-0.42

DepMap mean gene effect

-0.01

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• aflatoxin metabolic process
• alkaloid metabolic process
• cellular respiration
• cellular response to cadmium ion
• cholesterol metabolic process
• dibenzo-p-dioxin metabolic process
• epoxygenase P450 pathway
• estrogen metabolic process
• hydrogen peroxide biosynthetic process
• long-chain fatty acid biosynthetic process
• lung development
• methylation
• monocarboxylic acid metabolic process
• monoterpenoid metabolic process
• omega-hydroxylase P450 pathway
• oxidative demethylation
• porphyrin-containing compound metabolic process
• post-embryonic development
• regulation of gene expression
• retinol metabolic process
• steroid catabolic process
• toxin metabolic process
• xenobiotic catabolic process
• xenobiotic metabolic process
• toxin biosynthetic process

Molecular functions

• caffeine oxidase activity
• demethylase activity
• electron transfer activity
• enzyme binding
• estrogen 16-alpha-hydroxylase activity
• estrogen 2-hydroxylase activity
• heme binding
• hydroperoxy icosatetraenoate dehydratase activity
• iron ion binding
• monooxygenase activity
• oxidoreductase activity
• oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
• steroid hydroxylase activity

Cellular components

• endoplasmic reticulum membrane
• intracellular membrane-bounded organelle
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Cytochrome P450
• Cytochrome P450, E-class, group I
• Cytochrome P450, E-class, group I, CYP1
• Cytochrome P450, conserved site
• Cytochrome P450 superfamily
• Cytochrome P450

KeywordsUniProt

• Endoplasmic reticulum
• Fatty acid metabolism
• Glycoprotein
• Heme
• Iron
• Lipid metabolism
• Lyase
• Membrane
• Metal-binding
• Microsome
• Monooxygenase
• Oxidoreductase
• Steroid metabolism
• Sterol metabolism

InteractionsUniProt · HPA

Protein binding partners of CYP1A2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PGRMC1