CXCR3

Protein identityUniProt · HPA

UniProt accession

P49682

Gene

CXCR3

Ensembl

ENSG00000186810

Chromosome

X

Canonical length

368 aa

Protein class

CD markers, G-protein coupled receptors, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]

Canonical amino-acid sequenceUniProt

368 residues, UniProt reviewed canonical sequence.

>P49682|CXCR3
     1  MVLEVSDHQV LNDAEVAALL ENFSSSYDYG ENESDSCCTS PPCPQDFSLN FDRAFLPALY
    61  SLLFLLGLLG NGAVAAVLLS RRTALSSTDT FLLHLAVADT LLVLTLPLWA VDAAVQWVFG
   121  SGLCKVAGAL FNINFYAGAL LLACISFDRY LNIVHATQLY RRGPPARVTL TCLAVWGLCL
   181  LFALPDFIFL SAHHDERLNA THCQYNFPQV GRTALRVLQL VAGFLLPLLV MAYCYAHILA
   241  VLLVSRGQRR LRAMRLVVVV VVAFALCWTP YHLVVLVDIL MDLGALARNC GRESRVDVAK
   301  SVTSGLGYMH CCLNPLLYAF VGVKFRERMW MLLLRLGCPN QRGLQRQPSS SRRDSSWSET
   361  SEASYSGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CXCR3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

7

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

15 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CXCR3.

Disease | ImmuneIEDB

Conditions an epitope on CXCR3 was assayed in.

• systemic scleroderma B cell

ReferencesPubMed · IEDB

Publications for CXCR3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.97

gnomAD pLI

0.18

gnomAD missense Z

1.74

DepMap mean gene effect

0

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• adenylate cyclase-activating G protein-coupled receptor signaling pathway
• angiogenesis
• apoptotic process
• calcium-mediated signaling
• cell adhesion
• cell chemotaxis
• cell surface receptor signaling pathway
• chemotaxis
• G protein-coupled receptor signaling pathway
• immune response
• inflammatory response
• negative regulation of angiogenesis
• negative regulation of endothelial cell proliferation
• negative regulation of execution phase of apoptosis
• positive regulation of angiogenesis
• positive regulation of cell population proliferation
• positive regulation of chemotaxis
• positive regulation of cytosolic calcium ion concentration
• positive regulation of execution phase of apoptosis
• positive regulation of release of sequestered calcium ion into cytosol
• positive regulation of transcription by RNA polymerase II
• regulation of cell adhesion
• regulation of leukocyte migration
• T cell chemotaxis

Molecular functions

• C-C chemokine binding
• C-C chemokine receptor activity
• C-X-C chemokine binding
• C-X-C chemokine receptor activity
• chemokine binding
• chemokine receptor activity
• signaling receptor activity

Cellular components

• cell surface
• cytoplasm
• external side of plasma membrane
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• G protein-coupled receptor, rhodopsin-like
• Chemokine receptor family
• GPCR, rhodopsin-like, 7TM
• C-C chemokine receptor type 1-9-like
• 7 transmembrane receptor (rhodopsin family)
• CXC chemokine receptor 3

KeywordsUniProt

• Angiogenesis
• Apoptosis
• Cell membrane
• Chemotaxis
• Disulfide bond
• G-protein coupled receptor
• Glycoprotein
• Membrane
• Receptor
• Sulfation
• Transducer
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of CXCR3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CXCL10
• PF4