CXCL8

Protein identityUniProt · HPA

UniProt accession

P10145

Gene

CXCL8

Ensembl

ENSG00000169429

Chromosome

4

Canonical length

99 aa

Protein class

Cancer-related genes, Candidate cardiovascular disease genes, Plasma proteins, Predicted secreted proteins

Subcellular location

Golgi apparatus

Secretome location

Secreted to blood

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, May 2020]

Canonical amino-acid sequenceUniProt

99 residues, UniProt reviewed canonical sequence.

>P10145|CXCL8
     1  MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH
    61  CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CXCL8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.46

Highest tissue expression

2,989 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CXCL8.

Disease | AutoantibodyPubMed

Conditions in which antibodies against CXCL8 are reported. Each links to that disease's full target list.

Showing 0 of 1 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for CXCL8 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.86

gnomAD pLI

0

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• angiogenesis
• antimicrobial humoral immune response mediated by antimicrobial peptide
• calcium-mediated signaling
• cellular response to fibroblast growth factor stimulus
• cellular response to interleukin-1
• cellular response to lipopolysaccharide
• cellular response to tumor necrosis factor
• chemotaxis
• embryonic digestive tract development
• G protein-coupled receptor signaling pathway
• induction of positive chemotaxis
• inflammatory response
• intracellular signal transduction
• negative regulation of cell adhesion molecule production
• negative regulation of cell population proliferation
• negative regulation of G protein-coupled receptor signaling pathway
• negative regulation of gene expression
• neutrophil activation
• neutrophil chemotaxis
• positive regulation of angiogenesis
• positive regulation of gene expression
• positive regulation of neutrophil chemotaxis
• receptor internalization
• regulation of cell adhesion
• regulation of entry of bacterium into host cell
• response to endoplasmic reticulum stress
• response to molecule of bacterial origin
• signal transduction
• regulation of single stranded viral RNA replication via double stranded DNA intermediate

Molecular functions

• chemokine activity
• CXCR chemokine receptor binding
• heparin binding
• interleukin-8 receptor binding

Cellular components

• extracellular region
• extracellular space
• membrane

Protein domainsUniProt · Pfam · InterPro

• CXC chemokine
• Chemokine interleukin-8-like domain
• CXC chemokine, conserved site
• CXC Chemokine domain
• Chemokine interleukin-8-like superfamily
• Chemokine beta/gamma/delta
• Small cytokines (intecrine/chemokine), interleukin-8 like

KeywordsUniProt

• Chemotaxis
• Citrullination
• Cytokine
• Disulfide bond
• Inflammatory response
• Secreted
• Signal

InteractionsUniProt · HPA

Protein binding partners of CXCL8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TNFAIP6