CTPS2

Protein identityUniProt · HPA

UniProt accession

Q9NRF8

Gene

CTPS2

Ensembl

ENSG00000047230

Chromosome

X

Canonical length

586 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Mitochondria,Cytosol

Quaternary structure

Homotetramer

OverviewNCBI Gene

The protein encoded by this gene catalyzes the formation of CTP from UTP with the concomitant deamination of glutamine to glutamate. This protein is the rate-limiting enzyme in the synthesis of cytosine nucleotides, which play an important role in various metabolic processes and provide the precursors necessary for the synthesis of RNA and DNA. Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of this encoded protein. Thus, this protein is an attractive target for selective chemotherapy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Canonical amino-acid sequenceUniProt

586 residues, UniProt reviewed canonical sequence.

>Q9NRF8|CTPS2
     1  MKYILVTGGV ISGIGKGIIA SSIGTILKSC GLRVTAIKID PYINIDAGTF SPYEHGEVFV
    61  LNDGGEVDLD LGNYERFLDI NLYKDNNITT GKIYQHVINK ERRGDYLGKT VQVVPHITDA
   121  VQEWVMNQAK VPVDGNKEEP QICVIELGGT IGDIEGMPFV EAFRQFQFKA KRENFCNIHV
   181  SLVPQLSATG EQKTKPTQNS VRALRGLGLS PDLIVCRSST PIEMAVKEKI SMFCHVNPEQ
   241  VICIHDVSST YRVPVLLEEQ SIVKYFKERL HLPIGDSASN LLFKWRNMAD RYERLQKICS
   301  IALVGKYTKL RDCYASVFKA LEHSALAINH KLNLMYIDSI DLEKITETED PVKFHEAWQK
   361  LCKADGILVP GGFGIRGTLG KLQAISWART KKIPFLGVCL GMQLAVIEFA RNCLNLKDAD
   421  STEFRPNAPV PLVIDMPEHN PGNLGGTMRL GIRRTVFKTE NSILRKLYGD VPFIEERHRH
   481  RFEVNPNLIK QFEQNDLSFV GQDVDGDRME IIELANHPYF VGVQFHPEFS SRPMKPSPPY
   541  LGLLLAATGN LNAYLQQGCK LSSSDRYSDA SDDSFSEPRI AELEIS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CTPS2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

12 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.39

gnomAD pLI

0.82

gnomAD missense Z

2.59

DepMap mean gene effect

-0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• 'de novo' CTP biosynthetic process
• CTP biosynthetic process
• pyrimidine nucleobase biosynthetic process
• pyrimidine nucleotide metabolic process

Molecular functions

• ATP binding
• CTP synthase activity
• identical protein binding

Cellular components

• cytoophidium
• cytoplasm
• cytosol
• mitochondrion
• nucleus

Protein domainsUniProt · Pfam · InterPro

• CTP synthase
• CTP synthase, N-terminal
• Glutamine amidotransferase
• P-loop containing nucleoside triphosphate hydrolase
• Class I glutamine amidotransferase-like
• CTP synthase GATase domain
• Glutamine amidotransferase class-I
• CTP synthase N-terminus

KeywordsUniProt

• ATP-binding
• Cytoplasm
• Glutamine amidotransferase
• Ligase
• Magnesium
• Metal-binding
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Pyrimidine biosynthesis

InteractionsUniProt · HPA

Protein binding partners of CTPS2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CTPS1
• SPG21