Seroatlas · Human Serome Atlas

CSF3

Granulocyte colony-stimulating factor

Also known as: C17orf33, CSF3_HUMAN, G-CSF, GCSF, MGC45931

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P09919
Gene: CSF3
Ensembl: ENSG00000108342
Chromosome: 17
Canonical length: 207 aa
Protein class: Cancer-related genes, Plasma proteins, Predicted secreted proteins
Secretome location: Secreted to blood

OverviewNCBI Gene

This gene encodes a member of the IL-6 superfamily of cytokines. The encoded cytokine controls the production, differentiation, and function of granulocytes. Granulocytes are a type of white blood cell that are part of the innate immune response. A modified form of this protein is commonly administered to manage chemotherapy-induced neutropenia. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2020]

Canonical amino-acid sequenceUniProt

207 residues, UniProt reviewed canonical sequence.

>P09919|CSF3
     1  MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA
    61  LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG
   121  LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR
   181  AGGVLVASHL QSFLEVSYRV LRHLAQP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CSF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 142 nTPM

Expression across tissuesHPA

Tissue

lung: 142 nTPM
urinary bladder: 53 nTPM
adipose tissue: 45 nTPM
skeletal muscle: 24 nTPM
cervix: 24 nTPM
heart muscle: 18 nTPM

Single-cell type

endometrial secretory cells: 234 nCPM
vascular endothelial cells: 145 nCPM
myosatellite cells: 72 nCPM
alveolar cells type 2: 44 nCPM
fallopian secretory cells: 40 nCPM
conjunctival goblet cells: 23 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

medulla oblongata: 3 nTPM
spinal cord: 2.7 nTPM
cerebral cortex: 2.3 nTPM
pons: 2.3 nTPM
thalamus: 1.6 nTPM
amygdala: 1.5 nTPM

ReferencesPubMed · IEDB

Publications for CSF3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

3 publications

Autoantibodies against granulocyte colony-stimulating factor in Felty's syndrome and neutropenic systemic lupus erythematosus.
2002 · Arthritis Rheum · RCR 1.9 · 87 citations
Light chain (κ/λ) ratio of GM-CSF autoantibodies is associated with disease severity in autoimmune pulmonary alveolar proteinosis.
2013 · Clin Immunol · RCR 0.3 · 8 citations
Autoantibodies against granulocyte-macrophage colony stimulating factor and interleukin-3 are rare in patients with Felty's syndrome.
2004 · Ann Rheum Dis · RCR 0.2 · 8 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.69
gnomAD pLI: 0.38
gnomAD missense Z: 0.98
DepMap mean gene effect: -0.16
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

cytokine activity
enzyme binding
growth factor activity
granulocyte colony-stimulating factor receptor binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CSF3 as an antibody target. Whether an autoantibody or antibody against CSF3 could matter depends on whether native CSF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CSF3 is annotated as secreted, so native CSF3 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label CSF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...