Seroatlas · Human Serome Atlas

CRYBA4

Beta-crystallin A4

Also known as: CRBA4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P53673
Gene
CRYBA4
Ensembl
ENSG00000196431
Chromosome
22
Canonical length
196 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins

OverviewNCBI Gene

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

196 residues, UniProt reviewed canonical sequence.

>P53673|CRYBA4
     1  MTLQCTKSAG PWKMVVWDED GFQGRRHEFT AECPSVLELG FETVRSLKVL SGAWVGFEHA
    61  GFQGQQYILE RGEYPSWDAW GGNTAYPAER LTSFRPAACA NHRDSRLTIF EQENFLGKKG
   121  ELSDDYPSLQ AMGWEGNEVG SFHVHSGAWV CSQFPGYRGF QYVLECDHHS GDYKHFREWG
   181  SHAPTFQVQS IRRIQQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CRYBA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
1.4 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 1.4 nTPM
  • testis: 1.1 nTPM
  • duodenum: 0.2 nTPM
  • spleen: 0.2 nTPM
  • amygdala: 0.1 nTPM
  • appendix: 0.1 nTPM

Single-cell type

  • epididymal principal cells: 14 nCPM
  • undifferentiated spermatogonia: 5.5 nCPM
  • neutrophils: 5.3 nCPM
  • plasma cells: 4.8 nCPM
  • late primary spermatocytes: 3.8 nCPM
  • schwann cells: 2.1 nCPM

Immune cell

  • MAIT T-cell: 0.3 nTPM
  • NK-cell: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • white matter: 0.5 nTPM
  • cerebral cortex: 0.4 nTPM
  • amygdala: 0.2 nTPM
  • hippocampal formation: 0.2 nTPM
  • pons: 0.2 nTPM
  • thalamus: 0.2 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CRYBA4.

Disease | AllUniProt

Conditions CRYBA4 is implicated in, by any mechanism.

Disease | GeneticClinVar

6 pathogenic / likely-pathogenic of 109 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.39
gnomAD pLI
0
gnomAD missense Z
0.69
DepMap mean gene effect
-0.03
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CRYBA4 as an antibody target. Whether an autoantibody or antibody against CRYBA4 could matter depends on whether native CRYBA4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CRYBA4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CRYBA4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CRYBA4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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