Seroatlas · Human Serome Atlas

CLDN5

Claudin-5

Also known as: AWAL, BEC1, CLD5_HUMAN, CPETRL1, TMVCF

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O00501
Gene
CLDN5
Ensembl
ENSG00000184113
Chromosome
22
Canonical length
218 aa
Protein class
Predicted membrane proteins, Transporters

OverviewNCBI Gene

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]

Canonical amino-acid sequenceUniProt

218 residues, UniProt reviewed canonical sequence.

>O00501|CLDN5
     1  MGSAALEILG LVLCLVGWGG LILACGLPMW QVTAFLDHNI VTAQTTWKGL WMSCVVQSTG
    61  HMQCKVYDSV LALSTEVQAA RALTVSAVLL AFVALFVTLA GAQCTTCVAP GPAKARVALT
   121  GGVLYLFCGL LALVPLCWFA NIVVREFYDP SVPVSQKYEL GAALYIGWAA TALLMVGGCL
   181  LCCGAWVCTG RPDLSFPVKY SAPRRPTATG DYDKKNYV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CLDN5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
4
Mean surface accessibility (rSASA)
0.34
Highest tissue expression
424 nTPM

Expression across tissuesHPA

Tissue

  • adipose tissue: 424 nTPM
  • breast: 412 nTPM
  • choroid plexus: 407 nTPM
  • lung: 384 nTPM
  • midbrain: 189 nTPM
  • basal ganglia: 176 nTPM

Single-cell type

  • lymphatic endothelial cells: 1,227 nCPM
  • vascular endothelial cells: 711 nCPM
  • platelets: 627 nCPM
  • podocytes: 109 nCPM
  • megakaryocytes: 93 nCPM
  • respiratory ionocytes: 84 nCPM

Immune cell

  • total PBMC: 28 nTPM
  • basophil: 19 nTPM
  • neutrophil: 5.9 nTPM
  • naive CD4 T-cell: 4.8 nTPM
  • classical monocyte: 0.4 nTPM
  • memory CD8 T-cell: 0.3 nTPM

Brain region

  • choroid plexus: 330 nTPM
  • thalamus: 244 nTPM
  • pons: 170 nTPM
  • amygdala: 161 nTPM
  • medulla oblongata: 155 nTPM
  • midbrain: 138 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CLDN5.

Disease | GeneticClinVar

6 pathogenic / likely-pathogenic of 46 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.41
gnomAD pLI
0.9
gnomAD missense Z
1.44
DepMap mean gene effect
0.05
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CLDN5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CLDN5 as an antibody target. Whether an autoantibody or antibody against CLDN5 could matter depends on whether native CLDN5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CLDN5 is annotated at the cell surface, where native CLDN5 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label CLDN5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CLDN5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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