CIT

Protein identityUniProt · HPA

UniProt accession

O14578

Gene

CIT

Ensembl

ENSG00000122966

Chromosome

12

Canonical length

2027 aa

Protein class

Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

2027 residues, UniProt reviewed canonical sequence.

>O14578|CIT
     1  MLKFKYGARN PLDAGAAEPI ASRASRLNLF FQGKPPFMTQ QQMSPLSREG ILDALFVLFE
    61  ECSQPALMKI KHVSNFVRKY SDTIAELQEL QPSAKDFEVR SLVGCGHFAE VQVVREKATG
   121  DIYAMKVMKK KALLAQEQVS FFEEERNILS RSTSPWIPQL QYAFQDKNHL YLVMEYQPGG
   181  DLLSLLNRYE DQLDENLIQF YLAELILAVH SVHLMGYVHR DIKPENILVD RTGHIKLVDF
   241  GSAAKMNSNK MVNAKLPIGT PDYMAPEVLT VMNGDGKGTY GLDCDWWSVG VIAYEMIYGR
   301  SPFAEGTSAR TFNNIMNFQR FLKFPDDPKV SSDFLDLIQS LLCGQKERLK FEGLCCHPFF
   361  SKIDWNNIRN SPPPFVPTLK SDDDTSNFDE PEKNSWVSSS PCQLSPSGFS GEELPFVGFS
   421  YSKALGILGR SESVVSGLDS PAKTSSMEKK LLIKSKELQD SQDKCHKMEQ EMTRLHRRVS
   481  EVEAVLSQKE VELKASETQR SLLEQDLATY ITECSSLKRS LEQARMEVSQ EDDKALQLLH
   541  DIREQSRKLQ EIKEQEYQAQ VEEMRLMMNQ LEEDLVSARR RSDLYESELR ESRLAAEEFK
   601  RKATECQHKL LKAKDQGKPE VGEYAKLEKI NAEQQLKIQE LQEKLEKAVK ASTEATELLQ
   661  NIRQAKERAE RELEKLQNRE DSSEGIRKKL VEAEELEEKH REAQVSAQHL EVHLKQKEQH
   721  YEEKIKVLDN QIKKDLADKE TLENMMQRHE EEAHEKGKIL SEQKAMINAM DSKIRSLEQR
   781  IVELSEANKL AANSSLFTQR NMKAQEEMIS ELRQQKFYLE TQAGKLEAQN RKLEEQLEKI
   841  SHQDHSDKNR LLELETRLRE VSLEHEEQKL ELKRQLTELQ LSLQERESQL TALQAARAAL
   901  ESQLRQAKTE LEETTAEAEE EIQALTAHRD EIQRKFDALR NSCTVITDLE EQLNQLTEDN
   961  AELNNQNFYL SKQLDEASGA NDEIVQLRSE VDHLRREITE REMQLTSQKQ TMEALKTTCT
  1021  MLEEQVMDLE ALNDELLEKE RQWEAWRSVL GDEKSQFECR VRELQRMLDT EKQSRARADQ
  1081  RITESRQVVE LAVKEHKAEI LALQQALKEQ KLKAESLSDK LNDLEKKHAM LEMNARSLQQ
  1141  KLETERELKQ RLLEEQAKLQ QQMDLQKNHI FRLTQGLQEA LDRADLLKTE RSDLEYQLEN
  1201  IQVLYSHEKV KMEGTISQQT KLIDFLQAKM DQPAKKKKGL FSRRKEDPAL PTQVPLQYNE
  1261  LKLALEKEKA RCAELEEALQ KTRIELRSAR EEAAHRKATD HPHPSTPATA RQQIAMSAIV
  1321  RSPEHQPSAM SLLAPPSSRR KESSTPEEFS RRLKERMHHN IPHRFNVGLN MRATKCAVCL
  1381  DTVHFGRQAS KCLECQVMCH PKCSTCLPAT CGLPAEYATH FTEAFCRDKM NSPGLQTKEP
  1441  SSSLHLEGWM KVPRNNKRGQ QGWDRKYIVL EGSKVLIYDN EAREAGQRPV EEFELCLPDG
  1501  DVSIHGAVGA SELANTAKAD VPYILKMESH PHTTCWPGRT LYLLAPSFPD KQRWVTALES
  1561  VVAGGRVSRE KAEADAKLLG NSLLKLEGDD RLDMNCTLPF SDQVVLVGTE EGLYALNVLK
  1621  NSLTHVPGIG AVFQIYIIKD LEKLLMIAGE ERALCLVDVK KVKQSLAQSH LPAQPDISPN
  1681  IFEAVKGCHL FGAGKIENGL CICAAMPSKV VILRYNENLS KYCIRKEIET SEPCSCIHFT
  1741  NYSILIGTNK FYEIDMKQYT LEEFLDKNDH SLAPAVFAAS SNSFPVSIVQ VNSAGQREEY
  1801  LLCFHEFGVF VDSYGRRSRT DDLKWSRLPL AFAYREPYLF VTHFNSLEVI EIQARSSAGT
  1861  PARAYLDIPN PRYLGPAISS GAIYLASSYQ DKLRVICCKG NLVKESGTEH HRGPSTSRSS
  1921  PNKRGPPTYN EHITKRVASS PAPPEGPSHP REPSTPHRYR EGRTELRRDK SPGRPLEREK
  1981  SPGRMLSTRR ERSPGRLFED SSRGRLPAGA VRTPLSQVNK VWDQSSV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CIT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

13 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CIT.

Disease | AllUniProt

Conditions CIT is implicated in, by any mechanism.

• Microcephaly 17, primary, autosomal recessive (MCPH17) MIM:617090

Disease | GeneticClinVar

25 pathogenic / likely-pathogenic of 779 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Microcephaly 17, primary, autosomal recessive
• Autosomal recessive primary microcephaly
• Inborn genetic diseases

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.26

gnomAD pLI

1

gnomAD missense Z

3.82

DepMap mean gene effect

-0.5

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• generation of neurons
• mitotic cell cycle
• mitotic cytokinesis
• negative regulation of hippo signaling
• neuron apoptotic process
• positive regulation of cytokinesis
• regulation of actin cytoskeleton organization

Molecular functions

• ATP binding
• PDZ domain binding
• protein kinase binding
• protein serine kinase activity
• protein serine/threonine kinase activity
• protein serine/threonine kinase inhibitor activity
• scaffold protein binding
• SH3 domain binding
• transcription coactivator binding
• zinc ion binding

Cellular components

• cytosol
• membrane

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• AGC-kinase, C-terminal
• Citron homology (CNH) domain
• Pleckstrin homology domain
• Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• PH-like domain superfamily
• Protein kinase, ATP binding site
• Protein kinase, C-terminal
• C1-like domain superfamily
• Rho-associated Serine/Threonine Kinase
• Protein kinase domain
• PH domain
• Protein kinase C terminal domain
• CNH domain
• Citron Rho-interacting kinase
• Citron Rho-interacting kinase, catalytic domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cell cycle
• Cell division
• Coiled coil
• Cytoplasm
• Developmental protein
• Differentiation
• Kinase
• Metal-binding
• Mitosis
• Neurogenesis
• Nucleotide-binding
• Phosphoprotein
• Primary microcephaly
• Serine/threonine-protein kinase
• SH3-binding
• Transferase
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of CIT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TTC3
• PPP1R12A