Seroatlas · Human Serome Atlas

CHTF8

Chromosome transmission fidelity protein 8 homolog

Also known as: CTF8, CTF8_HUMAN, FLJ20400

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P0CG13
Gene: CHTF8
Ensembl: ENSG00000168802
Chromosome: 16
Canonical length: 121 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a short protein that forms part of the Ctf18 replication factor C (RFC) complex that occurs in both yeast and mammals. The heteroheptameric RFC complex plays a role in sister chromatid cohesion and may load the replication clamp PCNA (proliferating cell nuclear antigen) onto DNA during DNA replication and repair. This gene is ubiquitously expressed and has been shown to have reduced expression in renal and prostate tumors. Alternatively spliced transcript variants have been described. This gene has a pseudogene on chromosome X. [provided by RefSeq, Oct 2018]

Canonical amino-acid sequenceUniProt

121 residues, UniProt reviewed canonical sequence.

>P0CG13|CHTF8
     1  MVQIVISSAR AGGLAEWVLM ELQGEIEARY STGLAGNLLG DLHYTTEGIP VLIVGHHILY
    61  GKIIHLEKPF AVLVKHTPGD QDCDELGRET GTRYLVTALI KDKILFKTRP KPIITSVPKK
   121  V

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHTF8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 67 nTPM

Expression across tissuesHPA

Tissue

kidney: 67 nTPM
liver: 66 nTPM
pancreas: 47 nTPM
spleen: 43 nTPM
stomach: 43 nTPM
esophagus: 41 nTPM

Single-cell type

proximal tubule cells: 47 nCPM
choroid plexus epithelial cells: 40 nCPM
podocytes: 31 nCPM
renal collecting duct intercalated cells: 25 nCPM
distal convoluted tubule cells: 24 nCPM
loop of henle epithelial cells: 24 nCPM

Immune cell

basophil: 69 nTPM
non-classical monocyte: 39 nTPM
total PBMC: 39 nTPM
naive B-cell: 36 nTPM
classical monocyte: 35 nTPM
intermediate monocyte: 35 nTPM

Brain region

choroid plexus: 26 nTPM
white matter: 19 nTPM
basal ganglia: 19 nTPM
hippocampal formation: 18 nTPM
cerebral cortex: 18 nTPM
hypothalamus: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.51
gnomAD pLI: 0.83
gnomAD missense Z: 0.95
DepMap mean gene effect: -0.41
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

DNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Chromosome transmission fidelity protein 8
Ctf8

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CHTF8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHTF8 as an antibody target. Whether an autoantibody or antibody against CHTF8 could matter depends on whether native CHTF8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHTF8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHTF8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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