Seroatlas · Human Serome Atlas

CH25H

Cholesterol 25-hydroxylase

Also known as: CH25H_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O95992
Gene: CH25H
Ensembl: ENSG00000138135
Chromosome: 10
Canonical length: 272 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins

OverviewNCBI Gene

This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

272 residues, UniProt reviewed canonical sequence.

>O95992|CH25H
     1  MSCHNCSDPQ VLCSSGQLFL QPLWDHLRSW EALLQSPFFP VIFSITTYVG FCLPFVVLDI
    61  LCSWVPALRR YKIHPDFSPS AQQLLPCLGQ TLYQHVMFVF PVTLLHWARS PALLPHEAPE
   121  LLLLLHHILF CLLLFDMEFF VWHLLHHKVP WLYRTFHKVH HQNSSSFALA TQYMSVWELF
   181  SLGFFDMMNV TLLGCHPLTT LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD
   241  LHHSHFNCNF APYFTHWDKI LGTLRTASVP AR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CH25H can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 3
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 33 nTPM

Expression across tissuesHPA

Tissue

thymus: 33 nTPM
adipose tissue: 21 nTPM
urinary bladder: 17 nTPM
lung: 17 nTPM
esophagus: 14 nTPM
gallbladder: 14 nTPM

Single-cell type

vascular smooth muscle cells: 214 nCPM
pericytes: 150 nCPM
microglia: 101 nCPM
breast lactating cells: 97 nCPM
epididymal principal cells: 81 nCPM
fibroblasts: 48 nCPM

Immune cell

memory CD8 T-cell: 0.2 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

medulla oblongata: 30 nTPM
midbrain: 12 nTPM
spinal cord: 12 nTPM
hypothalamus: 12 nTPM
white matter: 11 nTPM
pons: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.87
gnomAD pLI: 0.23
gnomAD missense Z: 0.38
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

B cell chemotaxis
cholesterol metabolic process
lipid metabolic process
response to type I interferon
sterol biosynthetic process
negative regulation of cholesterol metabolic process
negative regulation of fusion of virus membrane with host plasma membrane

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CH25H as an antibody target. Whether an autoantibody or antibody against CH25H could matter depends on whether native CH25H is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CH25H is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CH25H as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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