Seroatlas · Human Serome Atlas

CES3

Carboxylesterase 3

Also known as: ES31, EST3_HUMAN, FLJ21736

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6UWW8
Gene
CES3
Ensembl
ENSG00000172828
Chromosome
16
Canonical length
571 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nuclear membrane,Endoplasmic reticulum
Secretome location
Intracellular and membrane

OverviewNCBI Gene

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.[provided by RefSeq, Jun 2010]

Canonical amino-acid sequenceUniProt

571 residues, UniProt reviewed canonical sequence.

>Q6UWW8|CES3
     1  MERAVRVESG VLVGVVCLLL ACPATATGPE VAQPEVDTTL GRVRGRQVGV KGTDRLVNVF
    61  LGIPFAQPPL GPDRFSAPHP AQPWEGVRDA STAPPMCLQD VESMNSSRFV LNGKQQIFSV
   121  SEDCLVLNVY SPAEVPAGSG RPVMVWVHGG ALITGAATSY DGSALAAYGD VVVVTVQYRL
   181  GVLGFFSTGD EHAPGNQGFL DVVAALRWVQ ENIAPFGGDL NCVTVFGGSA GGSIISGLVL
   241  SPVAAGLFHR AITQSGVITT PGIIDSHPWP LAQKIANTLA CSSSSPAEMV QCLQQKEGEE
   301  LVLSKKLKNT IYPLTVDGTV FPKSPKELLK EKPFHSVPFL MGVNNHEFSW LIPRGWGLLD
   361  TMEQMSREDM LAISTPVLTS LDVPPEMMPT VIDEYLGSNS DAQAKCQAFQ EFMGDVFINV
   421  PTVSFSRYLR DSGSPVFFYE FQHRPSSFAK IKPAWVKADH GAEGAFVFGG PFLMDESSRL
   481  AFPEATEEEK QLSLTMMAQW THFARTGDPN SKALPPWPQF NQAEQYLEIN PVPRAGQKFR
   541  EAWMQFWSET LPSKIQQWHQ KQKNRKAQED L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CES3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.24
Highest tissue expression
51 nTPM

Expression across tissuesHPA

Tissue

  • liver: 51 nTPM
  • colon: 36 nTPM
  • rectum: 30 nTPM
  • duodenum: 28 nTPM
  • skeletal muscle: 24 nTPM
  • small intestine: 22 nTPM

Single-cell type

  • colonocytes: 103 nCPM
  • breast lactating cells: 87 nCPM
  • enterocytes: 64 nCPM
  • hepatocytes: 64 nCPM
  • proximal tubule cells: 55 nCPM
  • enteric transient amplifying cells: 42 nCPM

Immune cell

  • eosinophil: 0.8 nTPM
  • neutrophil: 0.5 nTPM
  • basophil: 0.3 nTPM
  • naive CD4 T-cell: 0.2 nTPM
  • T-reg: 0.2 nTPM
  • classical monocyte: 0.1 nTPM

Brain region

  • cerebral cortex: 15 nTPM
  • hippocampal formation: 15 nTPM
  • pons: 15 nTPM
  • basal ganglia: 14 nTPM
  • midbrain: 13 nTPM
  • medulla oblongata: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.15
gnomAD pLI
0
gnomAD missense Z
0.53
DepMap mean gene effect
-0.11
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CES3 as an antibody target. Whether an autoantibody or antibody against CES3 could matter depends on whether native CES3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CES3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CES3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CES3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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