Seroatlas · Human Serome Atlas

CERS4

Ceramide synthase 4

Also known as: CERS4_HUMAN, FLJ12089, LASS4, Trh1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9HA82
Gene
CERS4
Ensembl
ENSG00000090661
Chromosome
19
Canonical length
394 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nucleoplasm,Vesicles

OverviewNCBI Gene

Enables sphingosine N-acyltransferase activity. Involved in ceramide biosynthetic process. Predicted to be located in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

394 residues, UniProt reviewed canonical sequence.

>Q9HA82|CERS4
     1  MLSSFNEWFW QDRFWLPPNV TWTELEDRDG RVYPHPQDLL AALPLALVLL AMRLAFERFI
    61  GLPLSRWLGV RDQTRRQVKP NATLEKHFLT EGHRPKEPQL SLLAAQCGLT LQQTQRWFRR
   121  RRNQDRPQLT KKFCEASWRF LFYLSSFVGG LSVLYHESWL WAPVMCWDRY PNQTLKPSLY
   181  WWYLLELGFY LSLLIRLPFD VKRKDFKEQV IHHFVAVILM TFSYSANLLR IGSLVLLLHD
   241  SSDYLLEACK MVNYMQYQQV CDALFLIFSF VFFYTRLVLF PTQILYTTYY ESISNRGPFF
   301  GYYFFNGLLM LLQLLHVFWS CLILRMLYSF MKKGQMEKDI RSDVEESDSS EEAAAAQEPL
   361  QLKNGAAGGP RPAPTDGPRS RVAGRLTNRH TTAT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CERS4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
6
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
84 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 84 nTPM
  • esophagus: 62 nTPM
  • epididymis: 58 nTPM
  • liver: 55 nTPM
  • salivary gland: 52 nTPM
  • thyroid gland: 50 nTPM

Single-cell type

  • esophageal apical cells: 283 nCPM
  • müller glia: 254 nCPM
  • esophageal suprabasal cells: 195 nCPM
  • breast lactating cells: 187 nCPM
  • respiratory ciliated cells: 179 nCPM
  • epididymal principal cells: 175 nCPM

Immune cell

  • eosinophil: 28 nTPM
  • neutrophil: 23 nTPM
  • NK-cell: 23 nTPM
  • memory B-cell: 20 nTPM
  • naive B-cell: 18 nTPM
  • basophil: 13 nTPM

Brain region

  • white matter: 96 nTPM
  • cerebral cortex: 86 nTPM
  • medulla oblongata: 84 nTPM
  • basal ganglia: 74 nTPM
  • amygdala: 71 nTPM
  • pons: 71 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.68
gnomAD pLI
0
gnomAD missense Z
-0.75
DepMap mean gene effect
-0.01
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CERS4 as an antibody target. Whether an autoantibody or antibody against CERS4 could matter depends on whether native CERS4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CERS4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CERS4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CERS4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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