CDK5RAP1

Protein identityUniProt · HPA

UniProt accession

Q96SZ6

Gene

CDK5RAP1

Ensembl

ENSG00000101391

Chromosome

20

Canonical length

601 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Mitochondria,Cytosol

OverviewNCBI Gene

This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013]

Canonical amino-acid sequenceUniProt

601 residues, UniProt reviewed canonical sequence.

>Q96SZ6|CDK5RAP1
     1  MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG ARKDFSSRLA
    61  AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR KVYLETYGCQ MNVNDTEIAW
   121  SILQKSGYLR TSNLQEADVI LLVTCSIREK AEQTIWNRLH QLKALKTRRP RSRVPLRIGI
   181  LGCMAERLKE EILNREKMVD ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP
   241  VQTSASATSA FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQVFLPPRPP
   301  KVLGLQGLKE VTLLGQNVNS FRDNSEVQFN SAVPTNLSRG FTTNYKTKQG GLRFAHLLDQ
   361  VSRVDPEMRI RFTSPHPKDF PDEVLQLIHE RDNICKQIHL PAQSGSSRVL EAMRRGYSRE
   421  AYVELVHHIR ESIPGVSLSS DFIAGFCGET EEDHVQTVSL LREVQYNMGF LFAYSMRQKT
   481  RAYHRLKDDV PEEVKLRRLE ELITIFREEA TKANQTSVGC TQLVLVEGLS KRSATDLCGR
   541  NDGNLKVIFP DAEMEDVNNP GLRVRAQPGD YVLVKITSAS SQTLRGHVLC RTTLRDSSAY
   601  C

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CDK5RAP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

21 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.36

gnomAD pLI

0

gnomAD missense Z

1.25

DepMap mean gene effect

0

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 17% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• brain development
• mitochondrial tRNA modification
• negative regulation of cell cycle
• negative regulation of cyclin-dependent protein serine/threonine kinase activity
• positive regulation of mitochondrial translation
• regulation of neuron differentiation
• RNA modification
• positive regulation of translational fidelity

Molecular functions

• 4 iron, 4 sulfur cluster binding
• metal ion binding
• protein kinase binding
• tRNA-2-methylthio-N(6)-dimethylallyladenosine(37) synthase activity

Cellular components

• cytosol
• mitochondrial matrix
• mitochondrion
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• TRAM domain
• Methylthiotransferase
• Elp3/MiaA/NifB-like, radical SAM core domain
• Radical SAM
• Methylthiotransferase, N-terminal
• Methylthiotransferase, conserved site
• Radical SAM, alpha/beta horseshoe
• Methylthiotransferase, N-terminal domain superfamily
• Radical SAM superfamily
• Uncharacterized protein family UPF0004
• TRAM domain
• Radical SAM superfamily
• tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB

KeywordsUniProt

• 4Fe-4S
• Iron
• Iron-sulfur
• Metal-binding
• Mitochondrion
• S-adenosyl-L-methionine
• Transferase
• Transit peptide
• tRNA processing

InteractionsUniProt · HPA

Protein binding partners of CDK5RAP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CDK5R1