CD47

Protein identityUniProt · HPA

UniProt accession

Q08722

Gene

CD47

Ensembl

ENSG00000196776

Chromosome

3

Canonical length

323 aa

Protein class

CD markers, Predicted intracellular proteins, Predicted membrane proteins, Transporters

Subcellular location

Vesicles,Plasma membrane

OverviewNCBI Gene

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

323 residues, UniProt reviewed canonical sequence.

>Q08722|CD47
     1  MWPLVAALLL GSACCGSAQL LFNKTKSVEF TFCNDTVVIP CFVTNMEAQN TTEVYVKWKF
    61  KGRDIYTFDG ALNKSTVPTD FSSAKIEVSQ LLKGDASLKM DKSDAVSHTG NYTCEVTELT
   121  REGETIIELK YRVVSWFSPN ENILIVIFPI FAILLFWGQF GIKTLKYRSG GMDEKTIALL
   181  VAGLVITVIV IVGAILFVPG EYSLKNATGL GLIVTSTGIL ILLHYYVFST AIGLTSFVIA
   241  ILVIQVIAYI LAVVGLSLCI AACIPMHGPL LISGLSILAL AQLLGLVYMK FVASNQKTIQ
   301  PPRKAVEEPL NAFKESKGMM NDE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CD47 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

5

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

264 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for CD47 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.37

gnomAD pLI

0.92

gnomAD missense Z

1.43

DepMap mean gene effect

0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• angiogenesis
• apoptotic process
• ATP export
• cell migration
• cellular response to interleukin-1
• cellular response to interleukin-12
• cellular response to type II interferon
• inflammatory response
• integrin-mediated signaling pathway
• negative regulation of phagocytosis
• positive regulation of cell population proliferation
• positive regulation of cell-cell adhesion
• positive regulation of inflammatory response
• positive regulation of monocyte extravasation
• positive regulation of phagocytosis
• positive regulation of stress fiber assembly
• positive regulation of T cell activation
• regulation of Fc receptor mediated stimulatory signaling pathway
• regulation of interleukin-10 production
• regulation of interleukin-12 production
• regulation of interleukin-6 production
• regulation of nitric oxide biosynthetic process
• regulation of tumor necrosis factor production
• regulation of type II interferon production

Molecular functions

• cell-cell adhesion mediator activity
• fibrinogen binding
• protein binding involved in heterotypic cell-cell adhesion
• thrombospondin receptor activity

Cellular components

• cell surface
• extracellular exosome
• plasma membrane
• specific granule membrane
• tertiary granule membrane

Protein domainsUniProt · Pfam · InterPro

• Immunoglobulin-like domain
• Immunoglobulin-like fold
• Immunoglobulin-like domain superfamily
• Leukocyte surface antigen CD47
• CD47-like, transmembrane
• CD47 immunoglobulin-like
• Leukocyte surface antigen CD47, IgV
• CD47 transmembrane region
• CD47 immunoglobulin-like domain

KeywordsUniProt

• Angiogenesis
• Apoptosis
• Cell adhesion
• Cell membrane
• Disulfide bond
• Glycoprotein
• Immunoglobulin domain
• Inflammatory response
• Membrane
• Phosphoprotein
• Pyrrolidone carboxylic acid
• Signal
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of CD47 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SIRPA
• SIRPG
• THBS1
• UBQLN1
• UBQLN2