Seroatlas · Human Serome Atlas

CAPN5

Calpain-5

Also known as: ADNIV, CAN5_HUMAN, HTRA3, nCL-3, VRNI

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O15484
Gene
CAPN5
Ensembl
ENSG00000149260
Chromosome
11
Canonical length
640 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

640 residues, UniProt reviewed canonical sequence.

>O15484|CAPN5
     1  MFSCVKPYED QNYSALRRDC RRRKVLFEDP LFPATDDSLY YKGTPGPAVR WKRPKGICED
    61  PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV IPDWKEQEWD PEKPNAYAGI
   121  FHFHFWRFGE WVDVVIDDRL PTVNNQLIYC HSNSRNEFWC ALVEKAYAKL AGCYQALDGG
   181  NTADALVDFT GGVSEPIDLT EGDFANDETK RNQLFERMLK VHSRGGLISA SIKAVTAADM
   241  EARLACGLVK GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERE WNGPWSDTSE
   301  EWQKVSKSER EKMGVTVQDD GEFWMTFEDV CRYFTDIIKC RVINTSHLSI HKTWEEARLH
   361  GAWTLHEDPR QNRGGGCINH KDTFFQNPQY IFEVKKPEDE VLICIQQRPK RSTRREGKGE
   421  NLAIGFDIYK VEENRQYRMH SLQHKAASSI YINSRSVFLR TDQPEGRYVI IPTTFEPGHT
   481  GEFLLRVFTD VPSNCRELRL DEPPHTCWSS LCGYPQLVTQ VHVLGAAGLK DSPTGANSYV
   541  IIKCEGDKVR SAVQKGTSTP EYNVKGIFYR KKLSQPITVQ VWNHRVLKDE FLGQVHLKAD
   601  PDNLQALHTL HLRDRNSRQP SNLPGTVAVH ILSSTSLMAV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CAPN5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.24
Highest tissue expression
57 nTPM

Expression across tissuesHPA

Tissue

  • duodenum: 57 nTPM
  • colon: 57 nTPM
  • liver: 51 nTPM
  • small intestine: 49 nTPM
  • rectum: 47 nTPM
  • stomach: 45 nTPM

Single-cell type

  • esophageal apical cells: 244 nCPM
  • colonocytes: 158 nCPM
  • foveolar cells: 156 nCPM
  • goblet cells: 145 nCPM
  • enterocytes: 90 nCPM
  • enteric transient amplifying cells: 65 nCPM

Immune cell

  • gdT-cell: 1.3 nTPM
  • naive CD8 T-cell: 0.8 nTPM
  • total PBMC: 0.7 nTPM
  • naive CD4 T-cell: 0.6 nTPM
  • memory CD8 T-cell: 0.5 nTPM
  • intermediate monocyte: 0.3 nTPM

Brain region

  • pons: 23 nTPM
  • medulla oblongata: 19 nTPM
  • spinal cord: 18 nTPM
  • hypothalamus: 15 nTPM
  • basal ganglia: 14 nTPM
  • cerebral cortex: 14 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CAPN5.

Disease | AllUniProt

Conditions CAPN5 is implicated in, by any mechanism.

  • Vitreoretinopathy, neovascular inflammatory (VRNI) MIM:193235

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 714 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Proliferative vitreoretinopathy
  • Autosomal dominant neovascular inflammatory vitreoretinopathy
  • Inborn genetic diseases
  • CAPN5-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.78
gnomAD pLI
0
gnomAD missense Z
0.53
DepMap mean gene effect
0.05
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CAPN5 as an antibody target. Whether an autoantibody or antibody against CAPN5 could matter depends on whether native CAPN5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CAPN5 is annotated at the cell surface, where native CAPN5 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label CAPN5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CAPN5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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