CA3

Protein identityUniProt · HPA

UniProt accession

P07451

Gene

CA3

Ensembl

ENSG00000164879

Chromosome

8

Canonical length

260 aa

Protein class

Enzymes, FDA approved drug targets, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]

Canonical amino-acid sequenceUniProt

260 residues, UniProt reviewed canonical sequence.

>P07451|CA3
     1  MAKEWGYASH NGPDHWHELF PNAKGENQSP VELHTKDIRH DPSLQPWSVS YDGGSAKTIL
    61  NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL
   121  VHWNPKYNTF KEALKQRDGI AVIGIFLKIG HENGEFQIFL DALDKIKTKG KEAPFTKFDP
   181  SCLFPACRDY WTYQGSFTTP PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL
   241  VSNWRPPQPI NNRVVRASFK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

4,942 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for CA3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.85

gnomAD pLI

0

gnomAD missense Z

0.55

DepMap mean gene effect

0.09

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to hypoxia
• cellular response to insulin stimulus
• cellular response to leptin stimulus
• liver regeneration
• negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
• negative regulation of reactive oxygen species biosynthetic process
• positive regulation of cell population proliferation
• response to bacterium
• response to ethanol
• response to lipid
• response to oxidative stress
• skeletal muscle contraction

Molecular functions

• carbonate dehydratase activity
• nickel cation binding
• phosphatase activity
• zinc ion binding

Cellular components

• cytoplasm
• cytosol
• nucleus
• sarcomere

Protein domainsUniProt · Pfam · InterPro

• Alpha carbonic anhydrase domain
• Carbonic anhydrase, alpha-class, conserved site
• Carbonic anhydrase, alpha-class
• Alpha carbonic anhydrase domain superfamily
• Eukaryotic-type carbonic anhydrase

KeywordsUniProt

• Acetylation
• Cytoplasm
• Glutathionylation
• Lyase
• Metal-binding
• Phosphoprotein
• Zinc