Seroatlas · Human Serome Atlas

B4GALNT4

N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1

Also known as: B4GN4_HUMAN, FLJ25045, NGalNAc-T1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q76KP1
Gene: B4GALNT4
Ensembl: ENSG00000182272
Chromosome: 11
Canonical length: 1039 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

Enables acetylgalactosaminyltransferase activity. Predicted to be located in Golgi cisterna membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1039 residues, UniProt reviewed canonical sequence.

>Q76KP1|B4GALNT4
     1  MPRLPVKKIR KQMKLLLLLL LLSCAAWLTY VHLGLVRQGR ALRQRLGYGR DGEKLTSETD
    61  GRGVHAAPST QRAEDSSESR EEEQAPEGRD LDMLFPGGAG RLPLNFTHQT PPWREEYKGQ
   121  VNLHVFEDWC GGAVGHLRRN LHFPLFPHTR TTVKKLAVSP KWKNYGLRIF GFIHPARDGD
   181  VQFSVASDDN SEFWLSLDES PAAAQLVAFV GKTGSEWTAP GEFTKFSSQV SKPRRLMASR
   241  RYYFELLHKQ DDRGSDHVEV GWRAFLPGLK FEVISSAHIS LYTDESALKM DHVAHVPQSP
   301  ASHVGGRPPQ EETSADMLRP DPRDTFFLTP RMESSSLENV LEPCAYAPTY VVKDFPIARY
   361  QGLQFVYLSF VYPNDYTRLT HMETDNKCFY RESPLYLERF GFYKYMKMDK EEGDEDEEDE
   421  VQRRAFLFLN PDDFLDDEDE GELLDSLEPT EAAPPRSGPQ SPAPAAPAQP GATLAPPTPP
   481  RPRDGGTPRH SRALSWAARA ARPLPLFLGR APPPRPAVEQ PPPKVYVTRV RPGQRASPRA
   541  PAPRAPWPPF PGVFLHPRPL PRVQLRAPPR PPRPHGRRTG GPQATQPRPP ARAQATQGGR
   601  EGQARTLGPA APTVDSNLSS EARPVTSFLS LSQVSGPQLP GEGEEEEEGE DDGAPGDEAA
   661  SEDSEEAAGP ALGRWREDAI DWQRTFSVGA VDFELLRSDW NDLRCNVSGN LQLPEAEAVD
   721  VTAQYMERLN ARHGGRFALL RIVNVEKRRD SARGSRFLLE LELQERGGGR LRLSEYVFLR
   781  LPGARVGDAD GESPEPAPAA SVRPDGRPEL CRPLRLAWRQ DVMVHFIVPV KNQARWVAQF
   841  LADMAALHAR TGDSRFSVVL VDFESEDMDV ERALRAARLP RYQYLRRTGN FERSAGLQAG
   901  VDAVEDASSI VFLCDLHIHF PPNILDGIRK HCVEGRLAFA PVVMRLSCGS SPRDPHGYWE
   961  VNGFGLFGIY KSDFDRVGGM NTEEFRDQWG GEDWELLDRV LQAGLEVERL RLRNFYHHYH
  1021  SKRGMWSVRS RKGSRTGAS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B4GALNT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 63 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 63 nTPM
cerebral cortex: 57 nTPM
amygdala: 46 nTPM
hippocampal formation: 39 nTPM
hypothalamus: 38 nTPM
basal ganglia: 38 nTPM

Single-cell type

tuft cells: 196 nCPM
astrocytes: 180 nCPM
bergmann glia: 56 nCPM
brain inhibitory neurons: 45 nCPM
müller glia: 44 nCPM
other brain neurons: 40 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebral cortex: 93 nTPM
amygdala: 80 nTPM
midbrain: 78 nTPM
medulla oblongata: 73 nTPM
hippocampal formation: 71 nTPM
basal ganglia: 68 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.58
gnomAD pLI: 0
gnomAD missense Z: 2.36
DepMap mean gene effect: -0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Golgi cisterna membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads B4GALNT4 as an antibody target. Whether an autoantibody or antibody against B4GALNT4 could matter depends on whether native B4GALNT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

B4GALNT4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label B4GALNT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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