AWAT1
Acyl-CoA wax alcohol acyltransferase 1
Also known as: AWAT1_HUMAN, DGAT2L3
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q58HT5
- Gene
- AWAT1
- Ensembl
- ENSG00000204195
- Chromosome
- X
- Canonical length
- 328 aa
- Protein class
- Enzymes, Metabolic proteins, Predicted membrane proteins
OverviewNCBI Gene
The protein encoded by this gene belongs to the diacylglycerol acyltransferase family. It esterifies long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that this enzyme plays a central role in lipid metabolism in skin. Consistent with this observation, this protein is predominantly expressed in the sebaceous gland of the skin. [provided by RefSeq, Sep 2009]
Canonical amino-acid sequenceUniProt
328 residues, UniProt reviewed canonical sequence.
>Q58HT5|AWAT1
1 MAHSKQPSHF QSLMLLQWPL SYLAIFWILQ PLFVYLLFTS LWPLPVLYFA WLFLDWKTPE
61 RGGRRSAWVR NWCVWTHIRD YFPITILKTK DLSPEHNYLM GVHPHGLLTF GAFCNFCTEA
121 TGFSKTFPGI TPHLATLSWF FKIPFVREYL MAKGVCSVSQ PAINYLLSHG TGNLVGIVVG
181 GVGEALQSVP NTTTLILQKR KGFVRTALQH GAHLVPTFTF GETEVYDQVL FHKDSRMYKF
241 QSCFRRIFGF YCCVFYGQSF CQGSTGLLPY SRPIVTVVGE PLPLPQIEKP SQEMVDKYHA
301 LYMDALHKLF DQHKTHYGCS ETQKLFFLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against AWAT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Other membrane
- Secreted
- No
- Transmembrane segments
- 2
- Mean surface accessibility (rSASA)
- 0.26
- Highest tissue expression
- 1.9 nTPM
Expression across tissuesHPA
Tissue
- skin: 1.9 nTPM
- breast: 0.6 nTPM
- adipose tissue: 0 nTPM
- adrenal gland: 0 nTPM
- amygdala: 0 nTPM
- appendix: 0 nTPM
Single-cell type
- hepatic stellate cells: 19 nCPM
- medullary thymic epithelial cells: 7.2 nCPM
- goblet cells: 6 nCPM
- adipocytes: 4 nCPM
- myonuclei: 2.7 nCPM
- müller glia: 2.3 nCPM
Immune cell
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
- MAIT T-cell: 0 nTPM
Brain region
- medulla oblongata: 0.9 nTPM
- amygdala: 0.8 nTPM
- thalamus: 0.8 nTPM
- white matter: 0.8 nTPM
- basal ganglia: 0.7 nTPM
- cerebral cortex: 0.7 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.19
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.26
- DepMap mean gene effect
- 0.15
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads AWAT1 as an antibody target. Whether an autoantibody or antibody against AWAT1 could matter depends on whether native AWAT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
AWAT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label AWAT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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