Seroatlas · Human Serome Atlas

ASNSD1

Asparagine synthetase domain-containing protein 1

Also known as: ASND1_HUMAN, FLJ20752, NBLA00058, NS3TP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NWL6
Gene: ASNSD1
Ensembl: ENSG00000138381
Chromosome: 2
Canonical length: 643 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

Predicted to enable asparagine synthase (glutamine-hydrolyzing) activity. Predicted to be involved in asparagine biosynthetic process. Predicted to act upstream of or within adipose tissue development; skeletal muscle tissue development; and transdifferentiation. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

643 residues, UniProt reviewed canonical sequence.

>Q9NWL6|ASNSD1
     1  MCGICCSVNF SAEHFSQDLK EDLLYNLKQR GPNSSKQLLK SDVNYQCLFS AHVLHLRGVL
    61  TTQPVEDERG NVFLWNGEIF SGIKVEAEEN DTQILFNYLS SCKNESEILS LFSEVQGPWS
   121  FIYYQASSHY LWFGRDFFGR RSLLWHFSNL GKSFCLSSVG TQTSGLANQW QEVPASGLFR
   181  IDLKSTVISG CIILQLYPWK YISRENIIEE NVNSLSQISA DLPAFVSVVA NEAKLYLEKP
   241  VVPLNMMLPQ AALETHCSNI SNVPPTREIL QVFLTDVHMK EVIQQFIDVL SVAVKKRVLC
   301  LPRDENLTAN EVLKTCDRKA NVAILFSGGI DSMVIATLAD RHIPLDEPID LLNVAFIAEE
   361  KTMPTTFNRE GNKQKNKCEI PSEEFSKDVA AAAADSPNKH VSVPDRITGR AGLKELQAVS
   421  PSRIWNFVEI NVSMEELQKL RRTRICHLIR PLDTVLDDSI GCAVWFASRG IGWLVAQEGV
   481  KSYQSNAKVV LTGIGADEQL AGYSRHRVRF QSHGLEGLNK EIMMELGRIS SRNLGRDDRV
   541  IGDHGKEARF PFLDENVVSF LNSLPIWEKA NLTLPRGIGE KLLLRLAAVE LGLTASALLP
   601  KRAMQFGSRI AKMEKINEKA SDKCGRLQIM SLENLSIEKE TKL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ASNSD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 66 nTPM

Expression across tissuesHPA

Tissue

tongue: 66 nTPM
skeletal muscle: 56 nTPM
liver: 38 nTPM
retina: 37 nTPM
bone marrow: 35 nTPM
ovary: 35 nTPM

Single-cell type

syncytiotrophoblasts: 113 nCPM
cytotrophoblasts: 57 nCPM
migrating cytotrophoblasts: 54 nCPM
megakaryocytes: 43 nCPM
paneth cells: 43 nCPM
parietal cells: 42 nCPM

Immune cell

eosinophil: 108 nTPM
basophil: 83 nTPM
T-reg: 58 nTPM
naive CD4 T-cell: 50 nTPM
NK-cell: 49 nTPM
neutrophil: 49 nTPM

Brain region

cerebellum: 45 nTPM
hypothalamus: 33 nTPM
white matter: 32 nTPM
cerebral cortex: 30 nTPM
spinal cord: 30 nTPM
pons: 30 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.47
gnomAD pLI: 0
gnomAD missense Z: 0.53
DepMap mean gene effect: -0.08
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

asparagine synthase (glutamine-hydrolyzing) activity

Protein domainsUniProt · Pfam · InterPro

Asparagine synthase
Rossmann-like alpha/beta/alpha sandwich fold
Glutamine amidotransferase type 2 domain
Nucleophile aminohydrolases, N-terminal
Asparagine synthase
Asparagine synthetase domain-containing protein

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ASNSD1 as an antibody target. Whether an autoantibody or antibody against ASNSD1 could matter depends on whether native ASNSD1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ASNSD1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ASNSD1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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