Seroatlas · Human Serome Atlas

ARHGEF33

Rho guanine nucleotide exchange factor 33

Also known as: ARG33_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A8MVX0
Gene: ARHGEF33
Ensembl: ENSG00000214694
Chromosome: 2
Canonical length: 844 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

Predicted to enable guanyl-nucleotide exchange factor activity. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

844 residues, UniProt reviewed canonical sequence.

>A8MVX0|ARHGEF33
     1  MEKTKTKQGE NEHMPVNNPS TQIYQLQALA SELKTGFTEA MQELSRIQHG EYALEEKVKS
    61  CRCSMEEKVT EMKNSLNYFK EELSNAMSMI QAITSKQEEM QQKIEQLQQE KRRESRKVKA
   121  KKTQKEEHSS QAGPAQAQGS PFRSINIPEP VLPSEDFTNL LPSQAYEKAQ ESRSVHVGDS
   181  NVKGMMGPGV NPTTPEAEEN LKSCLSADIQ SKGHLPSGMW RQPKDGKEWG EEYVTKDHPD
   241  KLKEAGQGRH SSLENVLCET SLAAKRQTVA LELLESERKY VINISLILKI KATFQGSDGK
   301  RNSKERSLFP GSLRYLVQQH LDLLHALQER VLKWPRQGVL GDLFLKLTND ENNFLDYYVA
   361  YLRDLPECIS LVHVVVLKEG DEEIKSDIYT LFFHIVQRIP EYLIHLQNVL KFTEQEHPDY
   421  YLLLVCVQRL RVFISHYTLL FQCNEDLLIQ KRKKLKKSSM AKLYKGLASQ CANAGQDASP
   481  TAGPEAVRDT GIHSEELLQP YPSAPSSGPA ITHLMPPVKK SQQQQSLMES MQPGKPSDWE
   541  LEGRKHERPE SLLAPTQFCA AEQDVKALAG PLQAIPEMDF ESSPAEPLGN VERSLRAPAE
   601  LLPDARGFVP AAYEEFEYGG EIFALPAPYD EEPFQAPALF ENCSPASSES SLDICFLRPV
   661  SFAMEAERPE HPLQPLPKSA TSPAGSSSAY KLEAAAQAHG KAKPLSRSLK EFPRAPPADG
   721  VAPRLYSTRS SSGGRAPIKA ERAAQAHGPA AAAVAARGAS RTFFPQQRSQ SEKQTYLEVR
   781  REMHLEDTTR FCPKEERESE QTSFSDQNPR QDQKGGFRSS FRKLFKKKNG NATGEDFCGP
   841  WGWW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGEF33 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.55
Highest tissue expression: 18 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 18 nTPM
testis: 10 nTPM
retina: 4.6 nTPM
basal ganglia: 4.5 nTPM
ovary: 4 nTPM
choroid plexus: 3.7 nTPM

Single-cell type

ependymal cells: 71 nCPM
late primary spermatocytes: 51 nCPM
oocytes: 46 nCPM
early primary spermatocytes: 41 nCPM
cardiomyocytes: 37 nCPM
early spermatids: 34 nCPM

Immune cell

eosinophil: 4.7 nTPM
intermediate monocyte: 0.4 nTPM
classical monocyte: 0.3 nTPM
MAIT T-cell: 0.1 nTPM
memory CD8 T-cell: 0.1 nTPM
naive B-cell: 0.1 nTPM

Brain region

cerebellum: 55 nTPM
thalamus: 18 nTPM
cerebral cortex: 15 nTPM
basal ganglia: 14 nTPM
midbrain: 14 nTPM
white matter: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.6
gnomAD pLI: 0
gnomAD missense Z: 1.7
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

guanyl-nucleotide exchange factor activity

Protein domainsUniProt · Pfam · InterPro

Dbl homology domain
Dbl homology (DH) domain superfamily
RhoGEF domain
Rho guanine nucleotide exchange factor 33

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGEF33 as an antibody target. Whether an autoantibody or antibody against ARHGEF33 could matter depends on whether native ARHGEF33 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGEF33 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGEF33 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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