Seroatlas · Human Serome Atlas

ARHGAP27

Rho GTPase-activating protein 27

Also known as: CAMGAP1, FLJ43547, RHG27_HUMAN, SH3D20, SH3P20

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6ZUM4
Gene: ARHGAP27
Ensembl: ENSG00000159314
Chromosome: 17
Canonical length: 889 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may pay a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2013]

Canonical amino-acid sequenceUniProt

889 residues, UniProt reviewed canonical sequence.

>Q6ZUM4|ARHGAP27
     1  MAADVVGDVY VLVEHPFEYT GKDGRRVAIR PNERYRLLRR STEHWWHVRR EPGGRPFYLP
    61  AQYVRELPAL GNPAAAAPPG PHPSPAAPEP LAYDYRFVSA AATAGPDGAP EESGGRASSL
   121  CGPAQRGAAT QRSSLAPGLP ACLYLRPAAP VRPAQSLNDL ACAAVSPPAG LLGSSGSFKA
   181  CSVAGSWVCP RPLARSDSEN VYEVIQDLHV PPPEESAEQV DDPPEPVYAN IERQPRATSP
   241  GAAAAPLPSP VWETHTDAGT GRPYYYNPDT GVTTWESPFE AAEGAASPAT SPASVDSHVS
   301  LETEWGQYWD EESRRVFFYN PLTGETAWED EAENEPEEEL EMQPGLSPGS PGDPRPPTPE
   361  TDYPESLTSY PEEDYSPVGS FGEPGPTSPL TTPPGWSCHV SQDKQMLYTN HFTQEQWVRL
   421  EDPHGKPYFY NPEDSSVRWE LPQVPVPAPR SIHKSSQDGD TPAQASPPEE KVPAELDEVG
   481  SWEEVSPATA AVRTKTLDKA GVLHRTKTAD KGKRLRKKHW SASWTVLEGG VLTFFKDSKT
   541  SAAGGLRQPS KFSTPEYTVE LRGATLSWAP KDKSSRKNVL ELRSRDGSEY LIQHDSEAII
   601  STWHKAIAQG IQELSAELPP EESESSRVDF GSSERLGSWQ EKEEDARPNA AAPALGPVGL
   661  ESDLSKVRHK LRKFLQRRPT LQSLREKGYI KDQVFGCALA ALCERERSRV PRFVQQCIRA
   721  VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA LKLFFRELPE
   781  PLFPFSHFRQ FIAAIKLQDQ ARRSRCVRDL VRSLPAPNHD TLRMLFQHLC RVIEHGEQNR
   841  MSVQSVAIVF GPTLLRPEVE ETSMPMTMVF QNQVVELILQ QCADIFPPH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP27 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 88 nTPM

Expression across tissuesHPA

Tissue

esophagus: 88 nTPM
spleen: 57 nTPM
small intestine: 48 nTPM
vagina: 39 nTPM
colon: 36 nTPM
cervix: 31 nTPM

Single-cell type

microglia: 78 nCPM
papillary tip epithelial cells: 38 nCPM
proximal tubule cells: 33 nCPM
renal collecting duct intercalated cells: 22 nCPM
renal collecting duct principal cells: 16 nCPM
renal connecting tubule cells: 16 nCPM

Immune cell

non-classical monocyte: 18 nTPM
intermediate monocyte: 14 nTPM
neutrophil: 13 nTPM
classical monocyte: 7.1 nTPM
eosinophil: 7 nTPM
total PBMC: 6.4 nTPM

Brain region

basal ganglia: 15 nTPM
thalamus: 10 nTPM
medulla oblongata: 9.3 nTPM
cerebral cortex: 8.7 nTPM
pons: 8.3 nTPM
midbrain: 8.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.57
gnomAD pLI: 0
gnomAD missense Z: 1.65
DepMap mean gene effect: -0.18
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARHGAP27 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

SH3KBP1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGAP27 as an antibody target. Whether an autoantibody or antibody against ARHGAP27 could matter depends on whether native ARHGAP27 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGAP27 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGAP27 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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