Seroatlas · Human Serome Atlas

ARHGAP12

Rho GTPase-activating protein 12

Also known as: FLJ10971, FLJ20737, FLJ21785, RHG12_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IWW6
Gene: ARHGAP12
Ensembl: ENSG00000165322
Chromosome: 10
Canonical length: 846 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may be involved in suppressing tumor formation by regulating cell invasion and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Canonical amino-acid sequenceUniProt

846 residues, UniProt reviewed canonical sequence.

>Q8IWW6|ARHGAP12
     1  MKMADRSGKI IPGQVYIEVE YDYEYEAKDR KIVIKQGERY ILVKKTNDDW WQVKPDENSK
    61  AFYVPAQYVK EVTRKALMPP VKQVAGLPNN STKIMQSLHL QRSTENVNKL PELSSFGKPS
   121  SSVQGTGLIR DANQNFGPSY NQGQTVNLSL DLTHNNGKFN NDSHSPKVSS QNRTRSFGHF
   181  PGPEFLDVEK TSFSQEQSCD SAGEGSERIH QDSESGDELS SSSTEQIRAT TPPNQGRPDS
   241  PVYANLQELK ISQSALPPLP GSPAIQINGE WETHKDSSGR CYYYNRGTQE RTWKPPRWTR
   301  DASISKGDFQ NPGDQELLSS EENYYSTSYS QSDSQCGSPP RGWSEELDER GHTLYTSDYT
   361  NEKWLKHVDD QGRQYYYSAD GSRSEWELPK YNASSQQQRE IIKSRSLDRR LQEPIVLTKW
   421  RHSTIVLDTN DKESPTASKP CFPENESSPS SPKHQDTASS PKDQEKYGLL NVTKIAENGK
   481  KVRKNWLSSW AVLQGSSLLF TKTQGSSTSW FGSNQSKPEF TVDLKGATIE MASKDKSSKK
   541  NVFELKTRQG TELLIQSDND TVINDWFKVL SSTINNQAVE TDEGIEEEIP DSPGIEKHDK
   601  EKEQKDPKKL RSFKVSSIDS SEQKKTKKNL KKFLTRRPTL QAVREKGYIK DQVFGSNLAN
   661  LCQRENGTVP KFVKLCIEHV EEHGLDIDGI YRVSGNLAVI QKLRFAVNHD EKLDLNDSKW
   721  EDIHVITGAL KMFFRELPEP LFTFNHFNDF VNAIKQEPRQ RVAAVKDLIR QLPKPNQDTM
   781  QILFRHLRRV IENGEKNRMT YQSIAIVFGP TLLKPEKETG NIAVHTVYQN QIVELILLEL
   841  SSIFGR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 28 nTPM

Expression across tissuesHPA

Tissue

stomach: 28 nTPM
spinal cord: 25 nTPM
pancreas: 20 nTPM
parathyroid gland: 18 nTPM
spleen: 17 nTPM
retina: 16 nTPM

Single-cell type

microglia: 469 nCPM
foveolar cells: 378 nCPM
choroid plexus epithelial cells: 255 nCPM
urothelial cells: 246 nCPM
fibro-adipogenic progenitors: 237 nCPM
gastric progenitor cells: 223 nCPM

Immune cell

NK-cell: 4.2 nTPM
memory CD8 T-cell: 3.3 nTPM
naive CD8 T-cell: 3.2 nTPM
naive CD4 T-cell: 3.1 nTPM
T-reg: 3.1 nTPM
gdT-cell: 3 nTPM

Brain region

white matter: 66 nTPM
medulla oblongata: 58 nTPM
pons: 53 nTPM
cerebellum: 49 nTPM
spinal cord: 49 nTPM
midbrain: 48 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.6
gnomAD pLI: 0
gnomAD missense Z: 1.04
DepMap mean gene effect: 0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

GTPase activator activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

Rho GTPase-activating protein domain
WW domain
SH3 domain
Pleckstrin homology domain
Rho GTPase activation protein
PH-like domain superfamily
WW domain superfamily
SH3-like domain superfamily
Rho GTPase-activating protein
SH3 domain
PH domain
WW domain
RhoGAP domain
ARHGAP12, SH3 domain
Linker region between SH3 and WW domains on ARHGAP12

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARHGAP12 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

ACTB

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGAP12 as an antibody target. Whether an autoantibody or antibody against ARHGAP12 could matter depends on whether native ARHGAP12 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGAP12 is annotated at the cell surface, where native ARHGAP12 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ARHGAP12 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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