Seroatlas · Human Serome Atlas

ARFGEF3

Brefeldin A-inhibited guanine nucleotide-exchange protein 3

Also known as: BIG3, BIG3_HUMAN, C6orf92, dJ171N11.1, KIAA1244, PPP1R33

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5TH69
Gene: ARFGEF3
Ensembl: ENSG00000112379
Chromosome: 6
Canonical length: 2177 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable guanyl-nucleotide exchange factor activity. Predicted to be involved in regulation of ARF protein signal transduction. Predicted to be located in transport vesicle membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

2177 residues, UniProt reviewed canonical sequence.

>Q5TH69|ARFGEF3
     1  MEEILRKLQK EASGSKYKAI KESCTWALET LGGLDTIVKI PPHVLREKCL LPLQLALESK
    61  NVKLAQHALA GMQKLLSEER FVSMETDSDE KQLLNQILNA VKVTPSLNED LQVEVMKVLL
   121  CITYTPTFDL NGSAVLKIAE VCIETYISSC HQRSINTAVR ATLSQMLSDL TLQLRQRQEN
   181  TIIENPDVPQ DFGNQGSTVE SLCDDVVSVL TVLCEKLQAA INDSQQLQLL YLECILSVLS
   241  SSSSSMHLHR RFTDLIWKNL CPALIVILGN PIHDKTITSA HTSSTSTSLE SDSASPGVSD
   301  HGRGSGCSCT APALSGPVAR TIYYIAAELV RLVGSVDSMK PVLQSLYHRV LLYPPPQHRV
   361  EAIKIMKEIL GSPQRLCDLA GPSSTESESR KRSISKRKSH LDLLKLIMDG MTEACIKGGI
   421  EACYAAVSCV CTLLGALDEL SQGKGLSEGQ VQLLLLRLEE LKDGAEWSRD SMEINEADFR
   481  WQRRVLSSEH TPWESGNERS LDISISVTTD TGQTTLEGEL GQTTPEDHSG NHKNSLKSPA
   541  IPEGKETLSK VLETEAVDQP DVVQRSHTVP YPDITNFLSV DCRTRSYGSR YSESNFSVDD
   601  QDLSRTEFDS CDQYSMAAEK DSGRSDVSDI GSDNCSLADE EQTPRDCLGH RSLRTAALSL
   661  KLLKNQEADQ HSARLFIQSL EGLLPRLLSL SNVEEVDTAL QNFASTFCSG MMHSPGFDGN
   721  SSLSFQMLMN ADSLYTAAHC ALLLNLKLSH GDYYRKRPTL APGVMKDFMK QVQTSGVLMV
   781  FSQAWIEELY HQVLDRNMLG EAGYWGSPED NSLPLITMLT DIDGLESSAI GGQLMASAAT
   841  ESPFAQSRRI DDSTVAGVAF ARYILVGCWK NLIDTLSTPL TGRMAGSSKG LAFILGAEGI
   901  KEQNQKERDA ICMSLDGLRK AARLSCALGV AANCASALAQ MAAASCVQEE KEEREAQEPS
   961  DAITQVKLKV EQKLEQIGKV QGVWLHTAHV LCMEAILSVG LEMGSHNPDC WPHVFRVCEY
  1021  VGTLEHNHFS DGASQPPLTI SQPQKATGSA GLLGDPECEG SPPEHSPEQG RSLSTAPVVQ
  1081  PLSIQDLVRE GSRGRASDFR GGSLMSGSSA AKVVLTLSTQ ADRLFEDATD KLNLMALGGF
  1141  LYQLKKASQS QLFHSVTDTV DYSLAMPGEV KSTQDRKSAL HLFRLGNAML RIVRSKARPL
  1201  LHVMRCWSLV APHLVEAACH KERHVSQKAV SFIHDILTEV LTDWNEPPHF HFNEALFRPF
  1261  ERIMQLELCD EDVQDQVVTS IGELVEVCST QIQSGWRPLF SALETVHGGN KSEMKEYLVG
  1321  DYSMGKGQAP VFDVFEAFLN TDNIQVFANA ATSYIMCLMK FVKGLGEVDC KEIGDCAPAP
  1381  GAPSTDLCLP ALDYLRRCSQ LLAKIYKMPL KPIFLSGRLA GLPRRLQEQS ASSEDGIESV
  1441  LSDFDDDTGL IEVWIILLEQ LTAAVSNCPR QHQPPTLDLL FELLRDVTKT PGPGFGIYAV
  1501  VHLLLPVMSV WLRRSHKDHS YWDMASANFK HAIGLSCELV VEHIQSFLHS DIRYESMINT
  1561  MLKDLFELLV ACVAKPTETI SRVGCSCIRY VLVTAGPVFT EEMWRLACCA LQDAFSATLK
  1621  PVKDLLGCFH SGTESFSGEG CQVRVAAPSS SPSAEAEYWR IRAMAQQVFM LDTQCSPKTP
  1681  NNFDHAQSCQ LIIELPPDEK PNGHTKKSVS FREIVVSLLS HQVLLQNLYD ILLEEFVKGP
  1741  SPGEEKTIQV PEAKLAGFLR YISMQNLAVI FDLLLDSYRT AREFDTSPGL KCLLKKVSGI
  1801  GGAANLYRQS AMSFNIYFHA LVCAVLTNQE TITAEQVKKV LFEDDERSTD SSQQCSSEDE
  1861  DIFEETAQVS PPRGKEKRQW RARMPLLSVQ PVSNADWVWL VKRLHKLCME LCNNYIQMHL
  1921  DLENCMEEPP IFKGDPFFIL PSFQSESSTP STGGFSGKET PSEDDRSQSR EHMGESLSLK
  1981  AGGGDLLLPP SPKVEKKDPS RKKEWWENAG NKIYTMAADK TISKLMTEYK KRKQQHNLSA
  2041  FPKEVKVEKK GEPLGPRGQD SPLLQRPQHL MDQGQMRHSF SAGPELLRQD KRPRSGSTGS
  2101  SLSVSVRDAE AQIQAWTNMV LTVLNQIQIL PDQTFTALQP AVFPCISQLT CHVTDIRVRQ
  2161  AVREWLGRVG RVYDIIV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARFGEF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 20 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 20 nTPM
salivary gland: 17 nTPM
parathyroid gland: 15 nTPM
cerebral cortex: 13 nTPM
prostate: 11 nTPM
spinal cord: 10 nTPM

Single-cell type

prostatic glandular cells: 626 nCPM
somatotrophs: 611 nCPM
salivary acinar cells: 548 nCPM
pancreatic islet cells: 490 nCPM
alveolar cells type 2: 482 nCPM
oligodendrocytes: 481 nCPM

Immune cell

basophil: 0.1 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

white matter: 88 nTPM
cerebellum: 74 nTPM
midbrain: 71 nTPM
cerebral cortex: 68 nTPM
hippocampal formation: 66 nTPM
basal ganglia: 65 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.2
gnomAD pLI: 1
DepMap mean gene effect: 0.09
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARFGEF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

PHB2

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARFGEF3 as an antibody target. Whether an autoantibody or antibody against ARFGEF3 could matter depends on whether native ARFGEF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARFGEF3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARFGEF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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