Seroatlas · Human Serome Atlas

ARFGAP3

ADP-ribosylation factor GTPase-activating protein 3

Also known as: ARFG3_HUMAN, ARFGAP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NP61
Gene
ARFGAP3
Ensembl
ENSG00000242247
Chromosome
22
Canonical length
516 aa
Protein class
Predicted intracellular proteins
Subcellular location
Golgi apparatus,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]

Canonical amino-acid sequenceUniProt

516 residues, UniProt reviewed canonical sequence.

>Q9NP61|ARFGAP3
     1  MGDPSKQDIL TIFKRLRSVP TNKVCFDCGA KNPSWASITY GVFLCIDCSG SHRSLGVHLS
    61  FIRSTELDSN WSWFQLRCMQ VGGNASASSF FHQHGCSTND TNAKYNSRAA QLYREKIKSL
   121  ASQATRKHGT DLWLDSCVVP PLSPPPKEED FFASHVSPEV SDTAWASAIA EPSSLTSRPV
   181  ETTLENNEGG QEQGPSVEGL NVPTKATLEV SSIIKKKPNQ AKKGLGAKKG SLGAQKLANT
   241  CFNEIEKQAQ AADKMKEQED LAKVVSKEES IVSSLRLAYK DLEIQMKKDE KMNISGKKNV
   301  DSDRLGMGFG NCRSVISHSV TSDMQTIEQE SPIMAKPRKK YNDDSDDSYF TSSSSYFDEP
   361  VELRSSSFSS WDDSSDSYWK KETSKDTETV LKTTGYSDRP TARRKPDYEP VENTDEAQKK
   421  FGNVKAISSD MYFGRQSQAD YETRARLERL SASSSISSAD LFEEPRKQPA GNYSLSSVLP
   481  NAPDMAQFKQ GVRSVAGKLS VFANGVVTSI QDRYGS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARFGAP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.55
Highest tissue expression
85 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 85 nTPM
  • adrenal gland: 84 nTPM
  • salivary gland: 65 nTPM
  • prostate: 61 nTPM
  • thyroid gland: 57 nTPM
  • cervix: 53 nTPM

Single-cell type

  • pancreatic acinar cells: 327 nCPM
  • neutrophils: 316 nCPM
  • prostatic glandular cells: 294 nCPM
  • endometrial glandular cells: 252 nCPM
  • salivary acinar cells: 249 nCPM
  • plasma cells: 245 nCPM

Immune cell

  • basophil: 87 nTPM
  • MAIT T-cell: 59 nTPM
  • plasmacytoid DC: 53 nTPM
  • non-classical monocyte: 51 nTPM
  • neutrophil: 51 nTPM
  • NK-cell: 50 nTPM

Brain region

  • choroid plexus: 37 nTPM
  • medulla oblongata: 32 nTPM
  • hypothalamus: 32 nTPM
  • white matter: 31 nTPM
  • thalamus: 29 nTPM
  • spinal cord: 29 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.94
gnomAD pLI
0
gnomAD missense Z
0.47
DepMap mean gene effect
0.04
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARFGAP3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARFGAP3 as an antibody target. Whether an autoantibody or antibody against ARFGAP3 could matter depends on whether native ARFGAP3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARFGAP3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARFGAP3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARFGAP3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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