Seroatlas · Human Serome Atlas

ANO8

Anoctamin-8

Also known as: ANO8_HUMAN, KIAA1623, TMEM16H

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HCE9
Gene: ANO8
Ensembl: ENSG00000074855
Chromosome: 19
Canonical length: 1232 aa
Protein class: Predicted membrane proteins

OverviewNCBI Gene

Enables intracellularly calcium-gated chloride channel activity. Involved in chloride transmembrane transport. Located in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1232 residues, UniProt reviewed canonical sequence.

>Q9HCE9|ANO8
     1  MAEAASGAGG TSLEGERGKR PPPEGEPAAP ASGVLDKLFG KRLLQAGRYL VSHKAWMKTV
    61  PTENCDVLMT FPDTTDDHTL LWLLNHIRVG IPELIVQVRH HRHTRAYAFF VTATYESLLR
   121  GADELGLRKA VKAEFGGGTR GFSCEEDFIY ENVESELRFF TSQERQSIIR FWLQNLRAKQ
   181  GEALHNVRFL EDQPIIPELA ARGIIQQVFP VHEQRILNRL MKSWVQAVCE NQPLDDICDY
   241  FGVKIAMYFA WLGFYTSAMV YPAVFGSVLY TFTEADQTSR DVSCVVFALF NVIWSTLFLE
   301  EWKRRGAELA YKWGTLDSPG EAVEEPRPQF RGVRRISPIT RAEEFYYPPW KRLLFQLLVS
   361  LPLCLACLVC VFLLMLGCFQ LQELVLSVKG LPRLARFLPK VMLALLVSVS AEGYKKLAIW
   421  LNDMENYRLE SAYEKHLIIK VVLFQFVNSY LSLFYIGFYL KDMERLKEML ATLLITRQFL
   481  QNVREVLQPH LYRRLGRGEL GLRAVWELAR ALLGLLSLRR PAPRRLEPQA DEGGGGGSGG
   541  GGRRCLSGGC GAPEEEEEAA LVERRRAGEG GEEGDGPPGG KEEDEDDEEE EDEEEEEDEE
   601  EGEEGGLLDC GLRLKKVSFA ERGAGRRRPG PSPEALLEEG SPTMVEKGLE PGVFTLAEED
   661  DEAEGAPGSP EREPPAILFR RAGGEGRDQG PDGGPDPEPG SNSDSTRRQR RQNRSSWIDP
   721  PEEEHSPQLT QAELESCMKK YEDTFQDYQE MFVQFGYVVL FSSAFPLAAL CALVNNLIEI
   781  RSDAFKLCTG LQRPFGQRVE SIGQWQKVME AMGVLAIVVN CYLIGQCGQL QRLFPWLSPE
   841  AAIVSVVVLE HFALLLKYLI HVAIPDIPGW VAEEMAKLEY QRREAFKRHE RQAQHRYQQQ
   901  QRRRREEEER QRHAEHHARR EHDSGGREEA RAEGSGLDPA TSSEKASAKA KGSTAGGHGP
   961  ERPKRPGSLL APNNVMKLKQ IIPLQGKFLS SGATSSLAAA GAGATTRPPP AQSPTGSDTR
  1021  LPAFLSFKFL KSPETRRDSE RSHSPPKAFH AGKLFPFGGT RAEPGSNGAG GQARPDGTPS
  1081  SGSSRVQRSG PVDEALAEEL EAPRPEEEGS GTALAPVGAP ALRTRRSRSP APPPPMPLPR
  1141  PPTPPAGCWQ WDGPWGCGGE GAAPRQALAA AECPPCAMAG PPPAPQPLPG DASFYSLPPP
  1201  PLPPTSDPLE TPAPSPSPSP SPQAVCWPSG WH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANO8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 8
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 27 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 27 nTPM
adrenal gland: 19 nTPM
cerebral cortex: 19 nTPM
skin: 17 nTPM
basal ganglia: 15 nTPM
amygdala: 14 nTPM

Single-cell type

astrocytes: 111 nCPM
esophageal apical cells: 47 nCPM
breast lactating cells: 44 nCPM
bergmann glia: 43 nCPM
adrenal cortex cells: 30 nCPM
ependymal cells: 25 nCPM

Immune cell

plasmacytoid DC: 0.2 nTPM
T-reg: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM

Brain region

cerebral cortex: 38 nTPM
medulla oblongata: 36 nTPM
thalamus: 34 nTPM
midbrain: 34 nTPM
pons: 33 nTPM
spinal cord: 32 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.32
gnomAD pLI: 0.96
gnomAD missense Z: 2.5
DepMap mean gene effect: -0.11
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANO8 as an antibody target. Whether an autoantibody or antibody against ANO8 could matter depends on whether native ANO8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANO8 is annotated at the cell surface, where native ANO8 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ANO8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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