Seroatlas · Human Serome Atlas

ANKRD66

Ankyrin repeat domain-containing protein 66

Also known as: ANR66_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
B4E2M5
Gene
ANKRD66
Ensembl
ENSG00000230062
Chromosome
6
Canonical length
196 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

No narrative summary is available for ANKRD66 in this catalog release; identity and structured annotations are shown without generated factual claims.

Canonical amino-acid sequenceUniProt

196 residues, UniProt reviewed canonical sequence.

>B4E2M5|ANKRD66
     1  MELAKMSDMT KLHQAVAAGD YSLVKKILKK GLCDPNYKDV DWNDRTPLHW AAIKGQMEVI
    61  RLLIEYGARP CLVTSVGWTP AHFAAEAGHL NILKTLHALH AAIDAPDFFG DTPKRIAQIY
   121  GQKACVAFLE KAEPECQDHR CAAQQKGLPL DERDEDWDAK KRELELSLPS LNQNMNKKNK
   181  KSRGPTRPSN TKGRRV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKRD66 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
38 nTPM

Expression across tissuesHPA

Tissue

  • fallopian tube: 38 nTPM
  • lung: 3 nTPM
  • basal ganglia: 1.4 nTPM
  • hippocampal formation: 1.4 nTPM
  • hypothalamus: 1.3 nTPM
  • amygdala: 0.8 nTPM

Single-cell type

  • fallopian tube ciliated cells: 243 nCPM
  • respiratory ciliated cells: 209 nCPM
  • epididymal efferent duct ciliated cells: 138 nCPM
  • endometrial ciliated cells: 115 nCPM
  • ependymal cells: 40 nCPM
  • respiratory deuterosomal cells: 3.1 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • midbrain: 11 nTPM
  • medulla oblongata: 9.8 nTPM
  • spinal cord: 6.8 nTPM
  • pons: 3.3 nTPM
  • white matter: 3.2 nTPM
  • hypothalamus: 3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.8
gnomAD pLI
0
gnomAD missense Z
0.95
DepMap mean gene effect
0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKRD66 as an antibody target. Whether an autoantibody or antibody against ANKRD66 could matter depends on whether native ANKRD66 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKRD66 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKRD66 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANKRD66. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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