Seroatlas · Human Serome Atlas

ANKRD65

Ankyrin repeat domain-containing protein 65

Also known as: ANR65_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
E5RJM6
Gene
ANKRD65
Ensembl
ENSG00000235098
Chromosome
1
Canonical length
399 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Microtubules,Mitotic spindle,Primary cilium,Primary cilium tip

OverviewNCBI Gene

No narrative summary is available for ANKRD65 in this catalog release; identity and structured annotations are shown without generated factual claims.

Canonical amino-acid sequenceUniProt

399 residues, UniProt reviewed canonical sequence.

>E5RJM6|ANKRD65
     1  MDSQRPEPRE EEEEEQELRW MELDSEEALG TRTEGPSVVQ GWGHLLQAVW RGPAGLVTQL
    61  LRQGASVEER DHAGRTPLHL AVLRGHAPLV RLLLQRGAPV GAVDRAGRTA LHEAAWHGHS
   121  RVAELLLQRG ASAAARSGTG LTPLHWAAAL GHTLLAARLL EAPGPGPAAA EAEDARGWTA
   181  AHWAAAGGRL AVLELLAAGG AGLDGALLVA AAAGRGAALR FLLARGARVD ARDGAGATAL
   241  GLAAALGRSQ DIEVLLGHGA DPGIRDRHGR SALHRAAARG HLLAVQLLVT QGAEVDARDT
   301  LGLTPLHHAS REGHVEVAGC LLDRGAQVDA TGWLRKTPLH LAAERGHGPT VGLLLSRGAS
   361  PTLRTQWAEV AQMPEGDLPQ ALPELGGGEK ECEGIESTG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKRD65 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
39 nTPM

Expression across tissuesHPA

Tissue

  • spinal cord: 39 nTPM
  • fallopian tube: 35 nTPM
  • amygdala: 28 nTPM
  • midbrain: 26 nTPM
  • hippocampal formation: 25 nTPM
  • cerebral cortex: 24 nTPM

Single-cell type

  • fallopian tube ciliated cells: 135 nCPM
  • breast myoepithelial cells: 84 nCPM
  • respiratory ciliated cells: 60 nCPM
  • prostatic hillock cells: 45 nCPM
  • fallopian secretory cells: 43 nCPM
  • smooth muscle cells: 42 nCPM

Immune cell

  • plasmacytoid DC: 7.9 nTPM
  • myeloid DC: 3.5 nTPM
  • naive B-cell: 0.3 nTPM
  • naive CD8 T-cell: 0.3 nTPM
  • classical monocyte: 0.2 nTPM
  • eosinophil: 0.2 nTPM

Brain region

  • white matter: 47 nTPM
  • medulla oblongata: 40 nTPM
  • pons: 36 nTPM
  • basal ganglia: 36 nTPM
  • midbrain: 34 nTPM
  • cerebellum: 28 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.96
gnomAD pLI
0
gnomAD missense Z
0.59
DepMap mean gene effect
-0.23
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKRD65 as an antibody target. Whether an autoantibody or antibody against ANKRD65 could matter depends on whether native ANKRD65 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKRD65 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKRD65 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANKRD65. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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