Seroatlas · Human Serome Atlas

ANKRD53

Ankyrin repeat domain-containing protein 53

Also known as: ANR53_HUMAN, FLJ12056, FLJ36160

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N9V6
Gene
ANKRD53
Ensembl
ENSG00000144031
Chromosome
2
Canonical length
530 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

Involved in mitotic metaphase chromosome alignment; regulation of microtubule cytoskeleton organization; and regulation of mitotic cytokinesis. Located in spindle pole. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

530 residues, UniProt reviewed canonical sequence.

>Q8N9V6|ANKRD53
     1  MASAGSTARR AGSGSWHSER GEGRGARPQP TPSGSMQQAN KVSLKATWTD AESKQPSQPL
    61  PDLADHLSAQ ATALARPRRP ASLTPPRADP SPSKESDQTA IDQTAIGSYY QLFAAAVGNV
   121  EWLRFCLNQS LREIPTDDKG FTAIHFAAQW GKLACLQVLV EEYKFPVDLL TNNSQTPLHL
   181  VIHRDNTTVA LPCIYYLLEK GADLNAQTCN GSTPLHLAAR DGLLDCVKVL VQSGANVHAQ
   241  DAMGYKPIDF CKIWNHRACA RFLKDAMWKK DKKDFAREMT KMKMFKSQLT LMEHNYLIEY
   301  QKEHKILREA AIRKWLHGKL HPGHSLVSNT KQARATALSK TPEQRESQRS RSFHPSVDAR
   361  LQCIPQPTEM PKPIYRKPTV KRPTMWNVSN NPARPPTTQI SHSQGIRLGV HPDPTPEHDF
   421  SSFLEVRPDG HGGARLHTVD GHWVAPVPRL PFEVLLRMLY PRVWPYRMKV PQGFYPISMR
   481  EVPRKRHLGD NTFWTDTLAM NLRDTFDEAF LAAVRSHQGL PTLPSPQTNP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKRD53 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.51
Highest tissue expression
25 nTPM

Expression across tissuesHPA

Tissue

  • testis: 25 nTPM
  • pancreas: 7.6 nTPM
  • adipose tissue: 7 nTPM
  • breast: 5.9 nTPM
  • retina: 5.7 nTPM
  • salivary gland: 4.3 nTPM

Single-cell type

  • late spermatids: 815 nCPM
  • early spermatids: 203 nCPM
  • rod photoreceptor cells: 80 nCPM
  • late primary spermatocytes: 48 nCPM
  • ependymal cells: 16 nCPM
  • plasma cells: 15 nCPM

Immune cell

  • plasmacytoid DC: 7.1 nTPM
  • basophil: 1.4 nTPM
  • memory CD8 T-cell: 0.2 nTPM
  • naive B-cell: 0.1 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM

Brain region

  • cerebellum: 5.8 nTPM
  • medulla oblongata: 4.3 nTPM
  • cerebral cortex: 3.8 nTPM
  • midbrain: 3.8 nTPM
  • spinal cord: 3.5 nTPM
  • amygdala: 3.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.37
gnomAD pLI
0
gnomAD missense Z
0.48
DepMap mean gene effect
-0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANKRD53 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKRD53 as an antibody target. Whether an autoantibody or antibody against ANKRD53 could matter depends on whether native ANKRD53 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKRD53 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKRD53 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANKRD53. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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