Seroatlas · Human Serome Atlas

ANKEF1

Ankyrin repeat and EF-hand domain-containing protein 1

Also known as: ANKE1_HUMAN, ANKRD5, dJ839B4.6, FLJ21669

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NU02
Gene: ANKEF1
Ensembl: ENSG00000132623
Chromosome: 20
Canonical length: 776 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli fibrillar center,Plasma membrane,Cytosol

OverviewNCBI Gene

Predicted to enable calcium ion binding activity. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

776 residues, UniProt reviewed canonical sequence.

>Q9NU02|ANKEF1
     1  MALADKRLEN LQIYKVLQCV RNKDKKQIEK LTKLGYPELI NYTEPINGLS ALHLASVSND
    61  IDMVSFLLDL GAHPDVQDRM GCTPTMRAAE LGHELSMEIL AKAKADMTIV DNEGKGVLFY
   121  CILPTKRHYR CALIALEHGA DVNNSTYEGK PIFLRACEDA HDVKDVCLTF LEKGANPNAI
   181  NSSTGRTALM EASREGVVEI VRGILERGGE VNAFDNDRHH AAHFAAKGGF FDILKLLFAY
   241  NGDVGLISIN GNTPLHYAAM GGFADCCKYI AQRGCDLKWK NLDHKTPRAV AKEGGFKAAS
   301  KEIRRAERIA NKLARPGAKN PNPLWALRLH DWSVEREAFL REAFAVLDRG DGSISKNDFV
   361  MVLEERQDYA SSEQLAAIAH LHEKTRGGGV NINEFFKGTR YLNKSFVLGS YGPKKKEKGM
   421  GKKGKKGKFV LPLPICVIPE YAFPRRQDGG PPYYMIETYK NVTDSSRFNR DHPPEHPIQD
   481  DSVWYIDDSE KVFSNINIIT KAGDLASLKK AFESGIPVDM KDNYYKTPLM TACASGNIDV
   541  VKFLLEKGAN VNATDNFLWT PLHFACHAGQ QDIVELLVES GALIDAASIN NSTPLNRAIE
   601  SCRLDTVKYL LDIGAKFQLE NRKGHSAMDV AKAYADYRII DLIKEKLDNL PKPAENQKLK
   661  GKTPPILKTE GPEIKKEEEL LSSIYGVPTT SEGKKVQKGN VVHLNSLITS GYTKKVDITF
   721  IPRRIWSPEA TTAELIRKRE LRRERFTHEV DFDDFMMPFQ KNITEKARAL EAALKT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKEF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 13 nTPM

Expression across tissuesHPA

Tissue

pancreas: 13 nTPM
thyroid gland: 5.4 nTPM
retina: 4.9 nTPM
testis: 4.3 nTPM
parathyroid gland: 4.1 nTPM
fallopian tube: 3.7 nTPM

Single-cell type

late spermatids: 553 nCPM
early spermatids: 430 nCPM
late primary spermatocytes: 47 nCPM
fallopian tube ciliated cells: 35 nCPM
respiratory ciliated cells: 24 nCPM
mucous neck cells: 22 nCPM

Immune cell

MAIT T-cell: 1.1 nTPM
naive CD4 T-cell: 1 nTPM
gdT-cell: 0.7 nTPM
memory CD4 T-cell: 0.7 nTPM
T-reg: 0.7 nTPM
naive CD8 T-cell: 0.6 nTPM

Brain region

choroid plexus: 8.8 nTPM
cerebellum: 8.5 nTPM
hypothalamus: 6.6 nTPM
thalamus: 6.3 nTPM
medulla oblongata: 6.1 nTPM
cerebral cortex: 5.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.94
gnomAD pLI: 0
gnomAD missense Z: 0.51
DepMap mean gene effect: 0.17
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

calcium ion binding

Protein domainsUniProt · Pfam · InterPro

EF-hand domain
Ankyrin repeat
EF-hand domain pair
Ankyrin repeat-containing domain superfamily
Ankyrin repeats (3 copies)
Ankyrin Repeat and EF-hand Domain-containing

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANKEF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

HIF1AN

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKEF1 as an antibody target. Whether an autoantibody or antibody against ANKEF1 could matter depends on whether native ANKEF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKEF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ANKEF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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