Seroatlas · Human Serome Atlas

ALPK3

Alpha-protein kinase 3

Also known as: ALPK3_HUMAN, KIAA1330, MAK, Midori

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96L96
Gene: ALPK3
Ensembl: ENSG00000136383
Chromosome: 15
Canonical length: 1705 aa
Protein class: Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable ATP binding activity; protein serine kinase activity; and protein serine/threonine kinase activity. Predicted to be involved in cardiac muscle cell development. Predicted to be active in nucleus. Implicated in hypertrophic cardiomyopathy 27. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1705 residues, UniProt reviewed canonical sequence.

>Q96L96|ALPK3
     1  MGSRRAPSRG WGAGGRSGAG GDGEDDGPVW IPSPASRSYL LSVRPETSLS SNRLSHPSSG
    61  RSTFCSIIAQ LTEETQPLFE TTLKSRSVSE DSDVRFTCIV TGYPEPEVTW YKDDTELDRY
   121  CGLPKYEITH QGNRHTLQLY RCREEDAAIY QASAQNSKGI VSCSGVLEVG TMTEYKIHQR
   181  WFAKLKRKAA AKLREIEQSW KHEKAVPGEV DTLRKLSPDR FQRKRRLSGA QAPGPSVPTR
   241  EPEGGTLAAW QEGETETAQH SGLGLINSFA SGEVTTNGEA APENGEDGEH GLLTYICDAM
   301  ELGPQRALKE ESGAKKKKKD EESKQGLRKP ELEKAAQSRR SSENCIPSSD EPDSCGTQGP
   361  VGVEQVQTQP RGRAARGPGS SGTDSTRKPA SAVGTPDKAQ KAPGPGPGQE VYFSLKDMYL
   421  ENTQAVRPLG EEGPQTLSVR APGESPKGKA PLRARSEGVP GAPGQPTHSL TPQPTRPFNR
   481  KRFAPPKPKG EATTDSKPIS SLSQAPECGA QSLGKAPPQA SVQVPTPPAR RRHGTRDSTL
   541  QGQAGHRTPG EVLECQTTTA PTMSASSSSD VASIGVSTSG SQGIIEPMDM ETQEDGRTSA
   601  NQRTGSKKNV QADGKIQVDG RTRGDGTQTA QRTRADRKTQ VDAGTQESKR PQSDRSAQKG
   661  MMTQGRAETQ LETTQAGEKI QEDRKAQADK GTQEDRRMQG EKGMQGEKGT QSEGSAPTAM
   721  EGQSEQEVAT SLGPPSRTPK LPPTAGPRAP LNIECFVQTP EGSCFPKKPG CLPRSEEAVV
   781  TASRNHEQTV LGPLSGNLML PAQPPHEGSV EQVGGERCRG PQSSGPVEAK QEDSPFQCPK
   841  EERPGGVPCM DQGGCPLAGL SQEVPTMPSL PGTGLTASPK AGPCSTPTSQ HGSTATFLPS
   901  EDQVLMSSAP TLHLGLGTPT QSHPPETMAT SSEGACAQVP DVEGRTPGPR SCDPGLIDSL
   961  KNYLLLLLKL SSTETSGAGG ESQVGAATGG LVPSATLTPT VEVAGLSPRT SRRILERVEN
  1021  NHLVQSAQTL LLSPCTSRRL TGLLDREVQA GRQALAAARG SWGPGPSSLT VPAIVVDEED
  1081  PGLASEGASE GEGEVSPEGP GLLGASQESS MAGRLGEAGG QAAPGQGPSA ESIAQEPSQE
  1141  EKFPGEALTG LPAATPEELA LGARRKRFLP KVRAAGDGEA TTPEERESPT VSPRGPRKSL
  1201  VPGSPGTPGR ERRSPTQGRK ASMLEVPRAE EELAAGDLGP SPKAGGLDTE VALDEGKQET
  1261  LAKPRKAKDL LKAPQVIRKI RVEQFPDASG SLKLWCQFFN ILSDSVLTWA KDQRPVGEVG
  1321  RSAGDEGPAA LAIVQASPVD CGVYRCTIHN EHGSASTDFC LSPEVLSGFI SREEGEVGEE
  1381  IEMTPMVFAK GLADSGCWGD KLFGRLVSEE LRGGGYGCGL RKASQAKVIY GLEPIFESGR
  1441  TCIIKVSSLL VFGPSSETSL VGRNYDVTIQ GCKIQNMSRE YCKIFAAEAR AAPGFGEVPE
  1501  IIPLYLIYRP ANNIPYATLE EDLGKPLESY CSREWGCAEA PTASGSSEAM QKCQTFQHWL
  1561  YQWTNGSFLV TDLAGVDWKM TDVQIATKLR GYQGLKESCF PALLDRFASS HQCNAYCELL
  1621  GLTPLKGPEA AHPQAKAKGS KSPSAGRKGS QLSPQPQKKG LPSPQGTRKS APSSKATPQA
  1681  SEPVTTQLLG QPPTQEEGSK AQGMR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALPK3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 198 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 198 nTPM
heart muscle: 62 nTPM
tongue: 55 nTPM
pituitary gland: 26 nTPM
blood vessel: 26 nTPM
adipose tissue: 12 nTPM

Single-cell type

myonuclei: 774 nCPM
somatotrophs: 229 nCPM
cardiomyocytes: 219 nCPM
lactotrophs: 194 nCPM
thymic myoid cells: 185 nCPM
thyrotrophs: 181 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 20 nTPM
pons: 11 nTPM
hypothalamus: 9 nTPM
midbrain: 8.3 nTPM
cerebral cortex: 7.9 nTPM
medulla oblongata: 5.7 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ALPK3.

Disease | AllUniProt

Conditions ALPK3 is implicated in, by any mechanism.

Cardiomyopathy, familial hypertrophic, 27 (CMH27) MIM:618052

Disease | GeneticClinVar

274 pathogenic / likely-pathogenic of 3,290 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Cardiovascular phenotype
Cardiomyopathy, familial hypertrophic 27
Cardiomyopathy
Hypertrophic cardiomyopathy
ALPK3-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.91
gnomAD pLI: 0
gnomAD missense Z: 0.13
DepMap mean gene effect: -0.18
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ALPK3 as an antibody target. Whether an autoantibody or antibody against ALPK3 could matter depends on whether native ALPK3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ALPK3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ALPK3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...