ALKBH1

Protein identityUniProt · HPA

UniProt accession

Q13686

Gene

ALKBH1

Ensembl

ENSG00000100601

Chromosome

14

Canonical length

389 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Endoplasmic reticulum

OverviewNCBI Gene

This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

389 residues, UniProt reviewed canonical sequence.

>Q13686|ALKBH1
     1  MGKMAAAVGS VATLATEPGE DAFRKLFRFY RQSRPGTADL EGVIDFSAAH AARGKGPGAQ
    61  KVIKSQLNVS SVSEQNAYRA GLQPVSKWQA YGLKGYPGFI FIPNPFLPGY QWHWVKQCLK
   121  LYSQKPNVCN LDKHMSKEET QDLWEQSKEF LRYKEATKRR PRSLLEKLRW VTVGYHYNWD
   181  SKKYSADHYT PFPSDLGFLS EQVAAACGFE DFRAEAGILN YYRLDSTLGI HVDRSELDHS
   241  KPLLSFSFGQ SAIFLLGGLQ RDEAPTAMFM HSGDIMIMSG FSRLLNHAVP RVLPNPEGEG
   301  LPHCLEAPLP AVLPRDSMVE PCSMEDWQVC ASYLKTARVN MTVRQVLATD QNFPLEPIED
   361  EKRDISTEGF CHLDDQNSEV KRARINPDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALKBH1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

6.9 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.03

gnomAD pLI

0

gnomAD missense Z

0.05

DepMap mean gene effect

-0.08

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• developmental growth
• DNA repair
• in utero embryonic development
• negative regulation of neuron apoptotic process
• neuron migration
• neuron projection development
• oxidative RNA demethylation
• placenta development
• positive regulation of gene expression, epigenetic
• regulation of mitochondrial translation
• regulation of translational elongation
• regulation of translational initiation
• RNA repair
• tRNA wobble cytosine modification
• regulation of translational initiation by tRNA modification

Molecular functions

• 2-oxoglutarate-dependent dioxygenase activity
• broad specificity oxidative DNA demethylase activity
• chemoattractant activity
• class I DNA-(apurinic or apyrimidinic site) endonuclease activity
• DNA N6-methyladenine demethylase activity
• ferrous iron binding
• oxidative RNA demethylase activity
• tRNA binding
• tRNA demethylase activity
• 2-oxoglutarate-dependent tRNA 5-methylcytidine formyltransferase activity

Cellular components

• cytoplasm
• endoplasmic reticulum
• euchromatin
• mitochondrial matrix
• mitochondrion
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Oxoglutarate/iron-dependent dioxygenase domain
• Alpha-ketoglutarate-dependent dioxygenase AlkB-like
• Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily
• 2OG-Fe(II) oxygenase superfamily
• Alkylated DNA repair protein AlkB

KeywordsUniProt

• Dioxygenase
• DNA damage
• DNA repair
• Iron
• Lyase
• Metal-binding
• Mitochondrion
• Multifunctional enzyme
• Nucleus
• Oxidoreductase
• RNA repair
• Transcription
• Transcription regulation
• Translation regulation

InteractionsUniProt · HPA

Protein binding partners of ALKBH1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DNAJB6