Seroatlas · Human Serome Atlas

AFAP1L2

Actin filament-associated protein 1-like 2

Also known as: AF1L2_HUMAN, Em:AC005383.4, FLJ14564, KIAA1914, XB130

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N4X5
Gene: AFAP1L2
Ensembl: ENSG00000169129
Chromosome: 10
Canonical length: 818 aa
Protein class: Predicted intracellular proteins
Subcellular location: Plasma membrane,Cytosol

OverviewNCBI Gene

Enables SH2 domain binding activity; SH3 domain binding activity; and protein tyrosine kinase activator activity. Involved in several processes, including positive regulation of epidermal growth factor receptor signaling pathway; regulation of gene expression; and regulation of mitotic cell cycle. Located in cytosol and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

818 residues, UniProt reviewed canonical sequence.

>Q8N4X5|AFAP1L2
     1  MERYKALEQL LTELDDFLKI LDQENLSSTA LVKKSCLAEL LRLYTKSSSS DEEYIYMNKV
    61  TINKQQNAES QGKAPEEQGL LPNGEPSQHS SAPQKSLPDL PPPKMIPERK QLAIPKTESP
   121  EGYYEEAEPY DTSLNEDGEA VSSSYESYDE EDGSKGKSAP YQWPSPEAGI ELMRDARICA
   181  FLWRKKWLGQ WAKQLCVIKD NRLLCYKSSK DHSPQLDVNL LGSSVIHKEK QVRKKEHKLK
   241  ITPMNADVIV LGLQSKDQAE QWLRVIQEVS GLPSEGASEG NQYTPDAQRF NCQKPDIAEK
   301  YLSASEYGSS VDGHPEVPET KDVKKKCSAG LKLSNLMNLG RKKSTSLEPV ERSLETSSYL
   361  NVLVNSQWKS RWCSVRDNHL HFYQDRNRSK VAQQPLSLVG CEVVPDPSPD HLYSFRILHK
   421  GEELAKLEAK SSEEMGHWLG LLLSESGSKT DPEEFTYDYV DADRVSCIVS AAKNSLLLMQ
   481  RKFSEPNTYI DGLPSQDRQE ELYDDVDLSE LTAAVEPTEE ATPVADDPNE RESDRVYLDL
   541  TPVKSFLHGP SSAQAQASSP TLSCLDNATE ALPADSGPGP TPDEPCIKCP ENLGEQQLES
   601  LEPEDPSLRI TTVKIQTEQQ RISFPPSCPD AVVATPPGAS PPVKDRLRVT SAEIKLGKNR
   661  TEAEVKRYTE EKERLEKKKE EIRGHLAQLR KEKRELKETL LKCTDKEVLA SLEQKLKEID
   721  EECRGEESRR VDLELSIMEV KDNLKKAEAG PVTLGTTVDT THLENVSPRP KAVTPASAPD
   781  CTPVNSATTL KNRPLSVVVT GKGTVLQKAK EWEKKGAS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AFAP1L2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.53
Highest tissue expression: 77 nTPM

Expression across tissuesHPA

Tissue

thyroid gland: 77 nTPM
parathyroid gland: 65 nTPM
spleen: 61 nTPM
seminal vesicle: 23 nTPM
endometrium: 22 nTPM
fallopian tube: 22 nTPM

Single-cell type

tuft cells: 265 nCPM
oligodendrocyte progenitor cells: 214 nCPM
renal collecting duct principal cells: 136 nCPM
schwann cells: 131 nCPM
podocytes: 127 nCPM
renal connecting tubule cells: 113 nCPM

Immune cell

T-reg: 1.8 nTPM
memory B-cell: 1.6 nTPM
naive B-cell: 1.6 nTPM
memory CD8 T-cell: 1.2 nTPM
MAIT T-cell: 1.1 nTPM
memory CD4 T-cell: 1.1 nTPM

Brain region

cerebellum: 33 nTPM
amygdala: 25 nTPM
spinal cord: 21 nTPM
thalamus: 20 nTPM
pons: 20 nTPM
white matter: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.49
gnomAD pLI: 0
gnomAD missense Z: 0.65
DepMap mean gene effect: 0.08
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of AFAP1L2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AFAP1L2 as an antibody target. Whether an autoantibody or antibody against AFAP1L2 could matter depends on whether native AFAP1L2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AFAP1L2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AFAP1L2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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