Seroatlas · Human Serome Atlas

AFAP1L1

Actin filament-associated protein 1-like 1

Also known as: AF1L1_HUMAN, FLJ36748

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8TED9
Gene: AFAP1L1
Ensembl: ENSG00000157510
Chromosome: 5
Canonical length: 768 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to be located in several cellular components, including actin cytoskeleton; anchoring junction; and cell projection. Predicted to be active in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

768 residues, UniProt reviewed canonical sequence.

>Q8TED9|AFAP1L1
     1  MDRGQVLEQL LPELTGLLSL LDHEYLSDTT LEKKMAVASI LQSLQPLPAK EVSYLYVNTA
    61  DLHSGPSFVE SLFEEFDCDL SDLRDMPEDD GEPSKGASPE LAKSPRLRNA ADLPPPLPNK
   121  PPPEDYYEEA LPLGPGKSPE YISSHNGCSP SHSIVDGYYE DADSSYPATR VNGELKSSYN
   181  DSDAMSSSYE SYDEEEEEGK SPQPRHQWPS EEASMHLVRE CRICAFLLRK KRFGQWAKQL
   241  TVIREDQLLC YKSSKDRQPH LRLALDTCSI IYVPKDSRHK RHELRFTQGA TEVLVLALQS
   301  REQAEEWLKV IREVSKPVGG AEGVEVPRSP VLLCKLDLDK RLSQEKQTSD SDSVGVGDNC
   361  STLGRRETCD HGKGKKSSLA ELKGSMSRAA GRKITRIIGF SKKKTLADDL QTSSTEEEVP
   421  CCGYLNVLVN QGWKERWCRL KCNTLYFHKD HMDLRTHVNA IALQGCEVAP GFGPRHPFAF
   481  RILRNRQEVA ILEASCSEDM GRWLGLLLVE MGSRVTPEAL HYDYVDVETL TSIVSAGRNS
   541  FLYARSCQNQ WPEPRVYDDV PYEKMQDEEP ERPTGAQVKR HASSCSEKSH RVDPQVKVKR
   601  HASSANQYKY GKNRAEEDAR RYLVEKEKLE KEKETIRTEL IALRQEKREL KEAIRSSPGA
   661  KLKALEEAVA TLEAQCRAKE ERRIDLELKL VAVKERLQQS LAGGPALGLS VSSKPKSGET
   721  ANKPQNSVPE QPLPVNCVSE LRKRSPSIVA SNQGRVLQKA KEWEMKKT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AFAP1L1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.52
Highest tissue expression: 30 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 30 nTPM
heart muscle: 24 nTPM
adipose tissue: 16 nTPM
tongue: 16 nTPM
placenta: 14 nTPM
breast: 9 nTPM

Single-cell type

lymphatic endothelial cells: 230 nCPM
early spermatids: 157 nCPM
vascular endothelial cells: 129 nCPM
extravillous trophoblasts: 109 nCPM
adipocytes: 90 nCPM
medullary thymic epithelial cells: 82 nCPM

Immune cell

basophil: 0.1 nTPM
neutrophil: 0.1 nTPM
NK-cell: 0.1 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM

Brain region

thalamus: 4.7 nTPM
amygdala: 4.2 nTPM
pons: 3.6 nTPM
cerebral cortex: 3.5 nTPM
medulla oblongata: 3.5 nTPM
choroid plexus: 3.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.89
gnomAD pLI: 0
gnomAD missense Z: 1.03
DepMap mean gene effect: 0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of AFAP1L1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

CTTN

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AFAP1L1 as an antibody target. Whether an autoantibody or antibody against AFAP1L1 could matter depends on whether native AFAP1L1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AFAP1L1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AFAP1L1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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