ADH4

Protein identityUniProt · HPA

UniProt accession

P08319

Gene

ADH4

Ensembl

ENSG00000198099

Chromosome

4

Canonical length

380 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

380 residues, UniProt reviewed canonical sequence.

>P08319|ADH4
     1  MGTKGKVIKC KAAIAWEAGK PLCIEEVEVA PPKAHEVRIQ IIATSLCHTD ATVIDSKFEG
    61  LAFPVIVGHE AAGIVESIGP GVTNVKPGDK VIPLYAPLCR KCKFCLSPLT NLCGKISNLK
   121  SPASDQQLME DKTSRFTCKG KPVYHFFGTS TFSQYTVVSD INLAKIDDDA NLERVCLLGC
   181  GFSTGYGAAI NNAKVTPGST CAVFGLGGVG LSAVMGCKAA GASRIIGIDI NSEKFVKAKA
   241  LGATDCLNPR DLHKPIQEVI IELTKGGVDF ALDCAGGSET MKAALDCTTA GWGSCTFIGV
   301  AAGSKGLTIF PEELIIGRTI NGTFFGGWKS VDSIPKLVTD YKNKKFNLDA LVTHTLPFDK
   361  ISEAFDLMNQ GKSVRTILIF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ADH4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.22

Highest tissue expression

4,816 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.27

gnomAD pLI

0

gnomAD missense Z

0.03

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• alcohol metabolic process
• aldehyde metabolic process
• ethanol metabolic process
• fatty acid omega-oxidation
• formaldehyde catabolic process
• retinoid metabolic process
• retinol metabolic process
• alcohol catabolic process
• quinone metabolic process

Molecular functions

• alcohol dehydrogenase (NAD+) activity
• aldose reductase (NADPH) activity
• all-trans retinal binding
• all-trans-retinol dehydrogenase (NAD+) activity
• benzaldehyde dehydrogenase (NAD+) activity
• NAD binding
• quinone reductase (NADPH) activity
• retinol binding
• S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity
• zinc ion binding

Cellular components

• cytosol
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• Alcohol dehydrogenase, zinc-type, conserved site
• GroES-like superfamily
• Alcohol dehydrogenase-like, C-terminal
• Alcohol dehydrogenase-like, N-terminal
• Enoylreductase domain
• NAD(P)-binding domain superfamily
• Zinc-binding dehydrogenase
• Alcohol dehydrogenase GroES-like domain

KeywordsUniProt

• Cytoplasm
• Lipid metabolism
• Metal-binding
• NAD
• Oxidoreductase
• Phosphoprotein
• Zinc