ADH1B

Protein identityUniProt · HPA

UniProt accession

P00325

Gene

ADH1B

Ensembl

ENSG00000196616

Chromosome

4

Canonical length

375 aa

Protein class

Enzymes, FDA approved drug targets, Human disease related genes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Plasma membrane,Primary cilium,Primary cilium transition zone,Basal body,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Canonical amino-acid sequenceUniProt

375 residues, UniProt reviewed canonical sequence.

>P00325|ADH1B
     1  MSTAGKVIKC KAAVLWEVKK PFSIEDVEVA PPKAYEVRIK MVAVGICHTD DHVVSGNLVT
    61  PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLFTPQCGK CRVCKNPESN YCLKNDLGNP
   121  RGTLQDGTRR FTCRGKPIHH FLGTSTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG
   181  YGSAVNVAKV TPGSTCAVFG LGGVGLSAVM GCKAAGAARI IAVDINKDKF AKAKELGATE
   241  CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPASQ
   301  NLSINPMLLL TGRTWKGAVY GGFKSKEGIP KLVADFMAKK FSLDALITHV LPFEKINEGF
   361  DLLHSGKSIR TVLTF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ADH1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.22

Highest tissue expression

5,476 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.24

gnomAD pLI

0

gnomAD missense Z

-0.38

DepMap mean gene effect

0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• retinoic acid metabolic process
• retinoid metabolic process
• retinol metabolic process

Molecular functions

• alcohol dehydrogenase (NAD+) activity
• all-trans-retinol dehydrogenase (NAD+) activity
• zinc ion binding

Cellular components

• ciliary basal body
• ciliary transition zone
• cilium
• cytosol
• nucleoplasm
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Alcohol dehydrogenase, zinc-type, conserved site
• GroES-like superfamily
• Alcohol dehydrogenase-like, C-terminal
• Alcohol dehydrogenase-like, N-terminal
• Enoylreductase domain
• NAD(P)-binding domain superfamily
• Zinc-binding dehydrogenase
• Alcohol dehydrogenase GroES-like domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Lipid metabolism
• Metal-binding
• NAD
• Oxidoreductase
• Phosphoprotein
• Zinc