Seroatlas · Human Serome Atlas

ACTL9

Actin-like protein 9

Also known as: ACTL9_HUMAN, MGC33407

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8TC94
Gene
ACTL9
Ensembl
ENSG00000181786
Chromosome
19
Canonical length
416 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Acrosome,Equatorial segment

OverviewNCBI Gene

Involved in acrosome assembly and fertilization. Located in acrosomal vesicle; perinuclear theca; and sperm head. Implicated in spermatogenic failure 53. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

416 residues, UniProt reviewed canonical sequence.

>Q8TC94|ACTL9
     1  MDASRPKSSE SQSSLEAPRP GPNPSPNVVN KPLQRDSPGM VADRLPPKTG AVVIDMGTGT
    61  CKVGFAGQAS PTYTVATILG CQPKKPATSG QSGLQTFIGE AARVLPELTL VQPLRSGIVV
   121  DWDAAELIWR HLLEHDLRVA THDHPLLFSD PPFSPATNRE KLVEVAFESL RSPAMYVASQ
   181  SVLSVYAHGR VSGLVVDTGH GVTYTVPVFQ GYNLLHATER LDLAGNNLTA FLAEMLLQAG
   241  LPLGQQDLDL VENIKHHYCY VASDFQKEQA RPEQEYKRTL KLPDGRTVTL GKELFQCPEL
   301  LFNPPEVPGL SPVGLSTMAK QSLRKLSLEM RADLAQNVLL CGGSSLFTGF EGRFRAELLR
   361  ALPAETHVVV AAQPTRNFSV WIGGSILASL RAFQSCWVLR EQYEEQGPYI VYRKCY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACTL9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
108 nTPM

Expression across tissuesHPA

Tissue

  • testis: 108 nTPM
  • adipose tissue: 0 nTPM
  • adrenal gland: 0 nTPM
  • amygdala: 0 nTPM
  • appendix: 0 nTPM
  • basal ganglia: 0 nTPM

Single-cell type

  • late spermatids: 3,435 nCPM
  • early spermatids: 1,199 nCPM
  • late primary spermatocytes: 221 nCPM
  • sertoli cells: 7.8 nCPM
  • leydig cells: 3.1 nCPM
  • undifferentiated spermatogonia: 3.1 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • amygdala: 0 nTPM
  • basal ganglia: 0 nTPM
  • cerebellum: 0 nTPM
  • cerebral cortex: 0 nTPM
  • choroid plexus: 0 nTPM
  • hippocampal formation: 0 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ACTL9.

Disease | AllUniProt

Conditions ACTL9 is implicated in, by any mechanism.

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 72 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Spermatogenic failure 53

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.14
gnomAD pLI
0
gnomAD missense Z
0.13
DepMap mean gene effect
-0.01
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ACTL9 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ACTL9 as an antibody target. Whether an autoantibody or antibody against ACTL9 could matter depends on whether native ACTL9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ACTL9 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ACTL9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ACTL9. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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