Seroatlas · Human Serome Atlas

ACTL8

Actin-like protein 8

Also known as: ACTL8_HUMAN, CT57

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H568
Gene: ACTL8
Ensembl: ENSG00000117148
Chromosome: 1
Canonical length: 366 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

Predicted to enable protein kinase binding activity. Predicted to be a structural constituent of postsynaptic actin cytoskeleton. Involved in epithelial cell differentiation. Predicted to be located in cytoskeleton. Predicted to be part of NuA4 histone acetyltransferase complex. Predicted to be active in several cellular components, including actin filament; axon; and synapse. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

366 residues, UniProt reviewed canonical sequence.

>Q9H568|ACTL8
     1  MAARTVIIDH GSGFLKAGTA GWNEPQMVFP NIVNYLPCKE NPGPSYARRR VSLGIDICHP
    61  DTFSYPIERG RILNWEGVQY LWSFVLENHR REQEVPPVII TETPLREPAD RKKMLEILFE
   121  LLHVPSVLLA DQLQMSLYAS GLLTGVVVDS GYGLTRVQPF HQGRPLPASG KTLEFAGQDL
   181  SAYLLKSLFK EDCDRRCLFQ LETVAVTQMN KCYVPQNLGE ALDFRERQQS ALDESNTYQL
   241  PDGSRVELTP MQRVAPEMFF SPQVFEQPGP SIPRAIVESV ESCEISLRPL LVSHVMACGG
   301  NTLYPGFTKR LFRELMGDHV SSTKATVWEG SNRNFSVWLG ASVVAHLSTY QSEWMSREEY
   361  GEHMRM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACTL8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 6.8 nTPM

Expression across tissuesHPA

Tissue

testis: 6.8 nTPM
ovary: 0.8 nTPM
colon: 0.2 nTPM
rectum: 0.2 nTPM
cerebral cortex: 0.1 nTPM
adipose tissue: 0 nTPM

Single-cell type

oocytes: 497 nCPM
differentiating spermatogonia: 88 nCPM
undifferentiated spermatogonia: 24 nCPM
early primary spermatocytes: 9 nCPM
early spermatids: 8 nCPM
gastric progenitor cells: 5.1 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

hypothalamus: 0.3 nTPM
cerebral cortex: 0.1 nTPM
white matter: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM
cerebellum: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.12
gnomAD pLI: 0.32
gnomAD missense Z: 0.88
DepMap mean gene effect: 0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ACTL8 as an antibody target. Whether an autoantibody or antibody against ACTL8 could matter depends on whether native ACTL8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ACTL8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ACTL8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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