Seroatlas · Human Serome Atlas

ZNF774

Zinc finger protein 774

Also known as: MGC75360, ZN774_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6NX45
Gene
ZNF774
Ensembl
ENSG00000196391
Chromosome
15
Canonical length
483 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Intermediate filaments,Cytosol

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

483 residues, UniProt reviewed canonical sequence.

>Q6NX45|ZNF774
     1  MWLGTSGKSG LPGHCLENPL QECHPAQLEE WALKGISRPS VISQPEQKEE PWVLPLQNFE
    61  ARKIPRESHT DCEHQVAKLN QDNSETAEQC GTSSERTNKD LSHTLSWGGN WEQGLELEGQ
   121  HGTLPGEGQL ESFSQERDLN KLLDGYVGEK PMCAECGKSF NQSSYLIRHL RTHTGERPYT
   181  CIECGKGFKQ SSDLVTHRRT HTGEKPYQCK GCEKKFSDSS TLIKHQRTHT GERPYECPEC
   241  GKTFGRKPHL IMHQRTHTGE KPYACLECHK SFSRSSNFIT HQRTHTGVKP YRCNDCGESF
   301  SQSSDLIKHQ RTHTGERPFK CPECGKGFRD SSHFVAHMST HSGERPFSCP DCHKSFSQSS
   361  HLVTHQRTHT GERPFKCENC GKGFADSSAL IKHQRIHTGE RPYKCGECGK SFNQSSHFIT
   421  HQRIHLGDRP YRCPECGKTF NQRSHFLTHQ RTHTGEKPFH CSKCNKSFRQ KAHLLCHQNT
   481  HLI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF774 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.46
Highest tissue expression
6.7 nTPM

Expression across tissuesHPA

Tissue

  • tongue: 6.7 nTPM
  • rectum: 6.6 nTPM
  • skeletal muscle: 5.7 nTPM
  • seminal vesicle: 5.4 nTPM
  • cerebellum: 4.8 nTPM
  • cerebral cortex: 4.5 nTPM

Single-cell type

  • myonuclei: 42 nCPM
  • adrenal cortex cells: 23 nCPM
  • early primary spermatocytes: 17 nCPM
  • late spermatids: 17 nCPM
  • late primary spermatocytes: 16 nCPM
  • brain inhibitory neurons: 16 nCPM

Immune cell

  • basophil: 1.3 nTPM
  • neutrophil: 1.1 nTPM
  • eosinophil: 0.7 nTPM
  • NK-cell: 0.7 nTPM
  • myeloid DC: 0.6 nTPM
  • naive B-cell: 0.4 nTPM

Brain region

  • cerebellum: 24 nTPM
  • basal ganglia: 24 nTPM
  • cerebral cortex: 22 nTPM
  • white matter: 22 nTPM
  • hypothalamus: 21 nTPM
  • amygdala: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.48
gnomAD pLI
0
gnomAD missense Z
0.17
DepMap mean gene effect
0.1
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF774 as an antibody target. Whether an autoantibody or antibody against ZNF774 could matter depends on whether native ZNF774 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF774 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF774 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ZNF774. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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