ZNF74

Protein identityUniProt · HPA

UniProt accession

Q16587

Gene

ZNF74

Ensembl

ENSG00000185252

Chromosome

22

Canonical length

644 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Actin filaments

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in actin cytoskeleton and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

644 residues, UniProt reviewed canonical sequence.

>Q16587|ZNF74
     1  MEIPAPEPEK TALSSQDPAL SLKENLEDIS GWGLPEARSK ESVSFKDVAV DFTQEEWGQL
    61  DSPQRALYRD VMLENYQNLL ALGPPLHKPD VISHLERGEE PWSMQREVPR GPCPEWELKA
   121  VPSQQQGICK EEPAQEPIME RPLGGAQAWG RQAGALQRSQ AAPWAPAPAM VWDVPVEEFP
   181  LRCPLFAQQR VPEGGPLLDT RKNVQATEGR TKAPARLCAG ENASTPSEPE KFPQVRRQRG
   241  AGAGEGEFVC GECGKAFRQS SSLTLHRRWH SREKAYKCDE CGKAFTWSTN LLEHRRIHTG
   301  EKPFFCGECG KAFSCHSSLN VHQRIHTGER PYKCSACEKA FSCSSLLSMH LRVHTGEKPY
   361  RCGECGKAFN QRTHLTRHHR IHTGEKPYQC GSCGKAFTCH SSLTVHEKIH SGDKPFKCSD
   421  CEKAFNSRSR LTLHQRTHTG EKPFKCADCG KGFSCHAYLL VHRRIHSGEK PFKCNECGKA
   481  FSSHAYLIVH RRIHTGEKPF DCSQCWKAFS CHSSLIVHQR IHTGEKPYKC SECGRAFSQN
   541  HCLIKHQKIH SGEKSFKCEK CGEMFNWSSH LTEHQRLHSE GKPLAIQFNK HLLSTYYVPG
   601  SLLGAGDAGL RDVDPIDALD VAKLLCVVPP RAGRNFSLGS KPRN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF74 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.52

Highest tissue expression

5.7 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.89

gnomAD pLI

0

gnomAD missense Z

1.14

DepMap mean gene effect

-0.03

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of DNA-templated transcription
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA binding
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• zinc ion binding

Cellular components

• actin cytoskeleton
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Krueppel-associated box
• Zinc finger C2H2-type
• Krueppel-associated box domain superfamily
• Zinc finger C2H2 superfamily
• Zinc finger, C2H2 type
• KRAB box

KeywordsUniProt

• DNA-binding
• Isopeptide bond
• Metal-binding
• Nucleus
• Repeat
• RNA-binding
• Transcription
• Transcription regulation
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of ZNF74 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TRIM28