Seroatlas · Human Serome Atlas

ZNF654

Zinc finger protein 654

Also known as: FLJ10997, FLJ21142, ZN654_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IZM8
Gene: ZNF654
Ensembl: ENSG00000175105
Chromosome: 3
Canonical length: 1128 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Microtubules,Cytokinetic bridge,Mitotic spindle

OverviewNCBI Gene

Predicted to enable DNA binding activity and DNA-binding transcription factor activity, RNA polymerase II-specific. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1128 residues, UniProt reviewed canonical sequence.

>Q8IZM8|ZNF654
     1  MAEEESDQEA ERLGEELVAI VESPLGPVGL RAAGDGRGGA GSGNCGGGVG ISSRDYCRRF
    61  CQVVEDYAGR WQVPLPQLQV LQTALCCFTT ASASFPDECE HVQYVLSSLA VSFFELLLFF
   121  GRDEFYEEPL KDILGSFQEC QNHLRRYGNV NLELVTRIIR DGGPWEDPVL QAVLKAQPAS
   181  QEIVNKYLSS ENPLFFELRA RYLIACERIP EAMALIKSCI NHPEISKDLY FHQALFTCLF
   241  MSPVEDQLFR EHLLKTDCKS GIDIICNAEK EGKTMLALQL CESFLIPQLQ NGDMYCIWEL
   301  IFIWSKLQLK SNPSKQVFVD QCYQLLRTAT NVRVIFPFMK IIKDEVEEEG LQICVEICGC
   361  ALQLDLHDDP KTKCLIYKTI AHFLPNDLEI LRICALSIFF LERSLEAYRT VEELYKRPDE
   421  EYNEGTSSVQ NRVRFELLPI LKKGLFFDPE FWNFVMIKKN CVALLSDKSA VRFLNESTLE
   481  NNAGNLKRTE EQQGLDEGFD SLTDQSTGET DPDDVSGVQP KGHINTKKNL TALSTSKVDH
   541  NVPRHRCMLC NKEFLGGHIV RHAQAHQKKG SFACVICGRK FRNRGLMQKH LKNHVKKIQR
   601  QQIAAAQQDD QEVTALEEIN CSSSSISFEN GNSDSKDLEV ETLTASSEGN KEVIPEHVAE
   661  FIEIPISVPE DVIENVIENG SPNNSLNNVF KPLTECGDDY EEEEDEEGDY EEDDYDLNQE
   721  TSVIHKINGT VCHPKDIYAT DQEGNFKCPA LGCVRIFKRI GFLNKHAMTV HPTDLNVRQT
   781  VMKWSKGKCK FCQRQFEDSQ HFIDHLNRHS YPNVYFCLHF NCNESFKLPF QLAQHTKSHR
   841  IFQAQCSFPE CHELFEDLPL LYEHEAQHYL SKTPESSAQP SETILWDVQT DSNPNQEKDS
   901  SSNEKQTISL PVSTSKSRKE STEPKTCIES MEKKTDSLVQ NGNERSDDTV SNISLIDQKM
   961  PDIEPNSENN CSSSDIVNGH SEIEQTPLVS SDPALKIDTN RIRTENGSIL PSVVPQEHNT
  1021  LPVSQAPSKP NLTSEHTSYG LILTKPYVRP LPPSYLDERY LSMPKRRKFL TDRVDACSDQ
  1081  DNVYKKSVKR LRCGKCLTTY CNAEALEAHL AQKKCQTLFG FDSDDESA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF654 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

retina: 11 nTPM
bone marrow: 7.6 nTPM
thymus: 7.1 nTPM
liver: 6.5 nTPM
skin: 6.1 nTPM
lymph node: 5.7 nTPM

Single-cell type

neutrophil progenitors: 161 nCPM
rod photoreceptor cells: 154 nCPM
hematopoietic stem cells: 150 nCPM
myonuclei: 149 nCPM
lactotrophs: 142 nCPM
neutrophils: 138 nCPM

Immune cell

basophil: 9.7 nTPM
NK-cell: 4.1 nTPM
memory CD8 T-cell: 2.4 nTPM
naive CD4 T-cell: 2.2 nTPM
MAIT T-cell: 2 nTPM
eosinophil: 1.8 nTPM

Brain region

cerebellum: 14 nTPM
white matter: 12 nTPM
hypothalamus: 11 nTPM
medulla oblongata: 11 nTPM
spinal cord: 10 nTPM
midbrain: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.17
gnomAD pLI: 1
gnomAD missense Z: 1.13
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of transcription by RNA polymerase II

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF654 as an antibody target. Whether an autoantibody or antibody against ZNF654 could matter depends on whether native ZNF654 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF654 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF654 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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