Seroatlas · Human Serome Atlas

ZNF653

Zinc finger protein 653

Also known as: Zip67, ZN653_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96CK0
Gene
ZNF653
Ensembl
ENSG00000161914
Chromosome
19
Canonical length
615 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Enables AF-2 domain binding activity; DNA-binding transcription factor binding activity; and transcription corepressor activity. Involved in extracellular negative regulation of signal transduction and negative regulation of transcription by RNA polymerase II. Predicted to be located in extracellular region. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

615 residues, UniProt reviewed canonical sequence.

>Q96CK0|ZNF653
     1  MAERALEPEA EAEAEAGAGG EAAAEEGAAG RKARGRPRLT ESDRARRRLE SRKKYDVRRV
    61  YLGEAHGPWV DLRRRSGWSD AKLAAYLISL ERGQRSGRHG KPWEQVPKKP KRKKRRRRNV
   121  NCLKNVVIWY EDHKHRCPYE PHLAELDPTF GLYTTAVWQC EAGHRYFQDL HSPLKPLSDS
   181  DPDSDKVGNG LVAGSSDSSS SGSASDSEES PEGQPVKAAA AAAAATPTSP VGSSGLITQE
   241  GVHIPFDVHH VESLAEQGTP LCSNPAGNGP EALETVVCVP VPVQVGAGPS ALFENVPQEA
   301  LGEVVASCPM PGMVPGSQVI IIAGPGYDAL TAEGIHLNMA AGSGVPGSGL GEEVPCAMME
   361  GVAAYTQTEP EGSQPSTMDA TAVAGIETKK EKEDLCLLKK EEKEEPVAPE LATTVPESAE
   421  PEAEADGEEL DGSDMSAIIY EIPKEPEKRR RSKRSRVMDA DGLLEMFHCP YEGCSQVYVA
   481  LSSFQNHVNL VHRKGKTKVC PHPGCGKKFY LSNHLRRHMI IHSGVREFTC ETCGKSFKRK
   541  NHLEVHRRTH TGETPLQCEI CGYQCRQRAS LNWHMKKHTA EVQYNFTCDR CGKRFEKLDS
   601  VKFHTLKSHP DHKPT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF653 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.62
Highest tissue expression
31 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 31 nTPM
  • testis: 24 nTPM
  • cerebral cortex: 17 nTPM
  • hippocampal formation: 15 nTPM
  • amygdala: 12 nTPM
  • hypothalamus: 12 nTPM

Single-cell type

  • late spermatids: 5 nCPM
  • respiratory ionocytes: 2.5 nCPM
  • late primary spermatocytes: 1.4 nCPM
  • fallopian tube ciliated cells: 1.2 nCPM
  • undifferentiated spermatogonia: 1.2 nCPM
  • respiratory ciliated cells: 1.1 nCPM

Immune cell

  • T-reg: 0.4 nTPM
  • plasmacytoid DC: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • cerebellum: 31 nTPM
  • cerebral cortex: 20 nTPM
  • hippocampal formation: 19 nTPM
  • hypothalamus: 18 nTPM
  • white matter: 16 nTPM
  • basal ganglia: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.67
gnomAD pLI
0
gnomAD missense Z
1.67
DepMap mean gene effect
-0.14
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZNF653 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF653 as an antibody target. Whether an autoantibody or antibody against ZNF653 could matter depends on whether native ZNF653 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF653 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF653 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ZNF653. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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