Seroatlas · Human Serome Atlas

ZNF548

Zinc finger protein 548

Also known as: FLJ32932, ZN548_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NEK5
Gene: ZNF548
Ensembl: ENSG00000188785
Chromosome: 19
Canonical length: 533 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

533 residues, UniProt reviewed canonical sequence.

>Q8NEK5|ZNF548
     1  MNLTEGRVVF EDVAIYFSQE EWGHLDEAQR LLYRDVMLEN LALLSSLGSW HGAEDEEAPS
    61  QQGFSVGVSE VTASKPCLSS QKVHPSETCG PPLKDILCLV EHNGIHPEQH IYICEAELFQ
   121  HPKQQIGENL SRGDDWIPSF GKNHRVHMAE EIFTCMEGWK DLPATSCLLQ HQGPQSEWKP
   181  YRDTEDREAF QTGQNDYKCS ECGKTFTCSY SFVEHQKIHT GERSYECNKC GKFFKYSANF
   241  MKHQTVHTSE RTYECRECGK SFMYNYRLMR HKRVHTGERP YECNTCGKFF RYSSTFVRHQ
   301  RVHTGERPYE CRECGKFFMD SSTLIKHQRV HTGERPYKCN DCGKFFRYIS TLIRHQRIHT
   361  GERPYECSVC GELFRYNSSL VKHWRNHTGE RPYKCSECGK SFRYHCRLIR HQRVHTGERP
   421  YECSECGKFF RYNSNLIKHW RNHTGERPYE CRECGKAFSH KHILVEHQKI HSGERPYECS
   481  ECQKAFIRKS HLVHHQKIHS EERLVCSMNV GNSLAKTPTS LNIRDFTMEK VYH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF548 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 25 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 25 nTPM
lymph node: 22 nTPM
spleen: 18 nTPM
tonsil: 17 nTPM
appendix: 16 nTPM
thymus: 12 nTPM

Single-cell type

gastric chief cells: 1.1 nCPM
breast myoepithelial cells: 0.9 nCPM
basal prostatic cells: 0.6 nCPM
epididymal basal cells: 0.5 nCPM
hematopoietic stem cells: 0.5 nCPM
innate lymphoid cells: 0.5 nCPM

Immune cell

naive B-cell: 8.4 nTPM
T-reg: 7 nTPM
naive CD8 T-cell: 5.8 nTPM
MAIT T-cell: 5.6 nTPM
naive CD4 T-cell: 5.5 nTPM
memory CD8 T-cell: 5.2 nTPM

Brain region

cerebellum: 36 nTPM
cerebral cortex: 35 nTPM
white matter: 27 nTPM
hippocampal formation: 27 nTPM
choroid plexus: 26 nTPM
hypothalamus: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.07
gnomAD pLI: 0.34
gnomAD missense Z: 0.41
DepMap mean gene effect: 0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of transcription by RNA polymerase II

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF548 as an antibody target. Whether an autoantibody or antibody against ZNF548 could matter depends on whether native ZNF548 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF548 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF548 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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