Seroatlas · Human Serome Atlas

ZNF543

Zinc finger protein 543

Also known as: DKFZp434H055, ZN543_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q08ER8
Gene
ZNF543
Ensembl
ENSG00000178229
Chromosome
19
Canonical length
600 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

600 residues, UniProt reviewed canonical sequence.

>Q08ER8|ZNF543
     1  MAASAQVSVT FEDVAVTFTQ EEWGQLDAAQ RTLYQEVMLE TCGLLMSLGC PLFKPELIYQ
    61  LDHRQELWMA TKDLSQSSYP GDNTKPKTTE PTFSHLALPE EVLLQEQLTQ GASKNSQLGQ
   121  SKDQDGPSEM QEVHLKIGIG PQRGKLLEKM SSERDGLGSD DGVCTKITQK QVSTEGDLYE
   181  CDSHGPVTDA LIREEKNSYK CEECGKVFKK NALLVQHERI HTQVKPYECT ECGKTFSKST
   241  HLLQHLIIHT GEKPYKCMEC GKAFNRRSHL TRHQRIHSGE KPYKCSECGK AFTHRSTFVL
   301  HHRSHTGEKP FVCKECGKAF RDRPGFIRHY IIHTGEKPYE CIECGKAFNR RSYLTWHQQI
   361  HTGVKPFECN ECGKAFCESA DLIQHYIIHT GEKPYKCMEC GKAFNRRSHL KQHQRIHTGE
   421  KPYECSECGK AFTHCSTFVL HKRTHTGEKP YECKECGKAF SDRADLIRHF SIHTGEKPYE
   481  CVECGKAFNR SSHLTRHQQI HTGEKPYECI QCGKAFCRSA NLIRHSIIHT GEKPYECSEC
   541  GKAFNRGSSL THHQRIHTGR NPTIVTDVGR PFMTAQTSVN IQELLLGKEF LNITTEENLW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF543 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
3.7 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 3.7 nTPM
  • testis: 3 nTPM
  • thymus: 3 nTPM
  • parathyroid gland: 2.9 nTPM
  • placenta: 2.6 nTPM
  • breast: 2.5 nTPM

Single-cell type

  • late spermatids: 20 nCPM
  • early spermatids: 19 nCPM
  • retinal horizontal cells: 14 nCPM
  • endometrial luminal cells: 8.4 nCPM
  • innate lymphoid cells: 7.7 nCPM
  • lactotrophs: 7.6 nCPM

Immune cell

  • eosinophil: 3.1 nTPM
  • memory B-cell: 2.3 nTPM
  • naive B-cell: 2.3 nTPM
  • memory CD8 T-cell: 2.2 nTPM
  • NK-cell: 2.1 nTPM
  • naive CD4 T-cell: 1.9 nTPM

Brain region

  • cerebellum: 18 nTPM
  • hypothalamus: 13 nTPM
  • cerebral cortex: 12 nTPM
  • white matter: 12 nTPM
  • basal ganglia: 12 nTPM
  • midbrain: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.68
gnomAD pLI
0
gnomAD missense Z
-0.59
DepMap mean gene effect
0.14
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZNF543 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF543 as an antibody target. Whether an autoantibody or antibody against ZNF543 could matter depends on whether native ZNF543 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF543 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF543 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ZNF543. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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