Seroatlas · Human Serome Atlas

ZNF474

Zinc finger protein 474

Also known as: 4933409D10Rik, FLJ32921, ZN474_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6S9Z5
Gene: ZNF474
Ensembl: ENSG00000164185
Chromosome: 5
Canonical length: 364 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear membrane,Plasma membrane

OverviewNCBI Gene

Predicted to enable zinc ion binding activity. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

364 residues, UniProt reviewed canonical sequence.

>Q6S9Z5|ZNF474
     1  MERGKKKRIS NKLQQTFHHS KEPTFLINQA GLLSSDSYSS LSPETESVNP GENIKTDTQK
    61  KRPGTVILSK LSSRRIISES QLSPPVIPAR RPGFRVCYIC GREFGSQSIA IHEPQCLQKW
   121  HIENSKLPKH LRRPEPSKPQ SLSSSGSYSL QATNEAAFQS AQAQLLPCES CGRTFLPDHL
   181  LVHHRSCKPK GEGPRAPHSN SSDHLTGLKK ACSGTPARPR TVICYICGKE FGTLSLPIHE
   241  PKCLEKWKME NDRLPVELHQ PLPQKPQPLP NAQSSQAGPN QAQLVFCPHC SRIFTSDRLL
   301  VHQRSCKTHP YGPKYQNLNL GSKGGLKEYT NSKQQRNRAA PSVTDKVIHA TQDALGEPGG
   361  ALCL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF474 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 16 nTPM

Expression across tissuesHPA

Tissue

fallopian tube: 16 nTPM
testis: 8.6 nTPM
choroid plexus: 7.6 nTPM
lung: 1.6 nTPM
thyroid gland: 0.8 nTPM
endometrium: 0.7 nTPM

Single-cell type

late spermatids: 589 nCPM
early spermatids: 113 nCPM
endometrial ciliated cells: 17 nCPM
late primary spermatocytes: 11 nCPM
fallopian tube ciliated cells: 9.4 nCPM
respiratory ciliated cells: 9.1 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 15 nTPM
midbrain: 9.2 nTPM
medulla oblongata: 5 nTPM
white matter: 4 nTPM
spinal cord: 3.7 nTPM
pons: 3.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.26
gnomAD pLI: 0
gnomAD missense Z: -1.53
DepMap mean gene effect: 0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

zinc ion binding

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF474 as an antibody target. Whether an autoantibody or antibody against ZNF474 could matter depends on whether native ZNF474 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF474 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF474 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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