Seroatlas · Human Serome Atlas

ZNF454

Zinc finger protein 454

Also known as: FLJ37444, ZN454_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N9F8
Gene: ZNF454
Ensembl: ENSG00000178187
Chromosome: 5
Canonical length: 522 aa
Protein class: Predicted intracellular proteins, Transcription factors

OverviewNCBI Gene

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

522 residues, UniProt reviewed canonical sequence.

>Q8N9F8|ZNF454
     1  MAVSHLPTMV QESVTFKDVA ILFTQEEWGQ LSPAQRALYR DVMLENYSNL VSLGLLGPKP
    61  DTFSQLEKRE VWMPEDTPGG FCLDWMTMPA SKKSTVKAEI PEEELDQWTI KERFSSSSHW
   121  KCASLLEWQC GGQEISLQRV VLTHPNTPSQ ECDESGSTMS SSLHSDQSQG FQPSKNAFEC
   181  SECGKVFSKS STLNKHQKIH NEKNANQKIH IKEKRYECRE CGKAFHQSTH LIHHQRIHTG
   241  EKPYECKECG KAFSVSSSLT YHQKIHTGEK PFECNLCGKA FIRNIHLAHH HRIHTGEKPF
   301  KCNICEKAFV CRAHLTKHQN IHSGEKPYKC NECGKAFNQS TSFLQHQRIH TGEKPFECNE
   361  CGKAFRVNSS LTEHQRIHTG EKPYKCNECG KAFRDNSSFA RHRKIHTGEK PYRCGLCEKA
   421  FRDQSALAQH QRIHTGEKPY TCNICEKAFS DHSALTQHKR IHTREKPYKC KICEKAFIRS
   481  THLTQHQRIH TGEKPYKCNK CGKAFNQTAN LIQHQRHHIG EK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF454 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 2.2 nTPM

Expression across tissuesHPA

Tissue

retina: 2.2 nTPM
pituitary gland: 2.1 nTPM
testis: 1.9 nTPM
ovary: 1.7 nTPM
fallopian tube: 1.6 nTPM
cerebellum: 1.5 nTPM

Single-cell type

early primary spermatocytes: 29 nCPM
lactotrophs: 23 nCPM
retinal bipolar cells: 22 nCPM
somatotrophs: 22 nCPM
gonadotrophs: 15 nCPM
thyrotrophs: 14 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebellum: 11 nTPM
hypothalamus: 8.4 nTPM
midbrain: 6.7 nTPM
basal ganglia: 6.5 nTPM
white matter: 6.4 nTPM
cerebral cortex: 6.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.92
gnomAD pLI: 0
gnomAD missense Z: 1.29
DepMap mean gene effect: 0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of transcription by RNA polymerase II

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF454 as an antibody target. Whether an autoantibody or antibody against ZNF454 could matter depends on whether native ZNF454 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF454 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF454 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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