Seroatlas · Human Serome Atlas

ZNF404

Zinc finger protein 404

Also known as: ZN404_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q494X3
Gene: ZNF404
Ensembl: ENSG00000176222
Chromosome: 19
Canonical length: 552 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoli,Nucleoli rim,Intermediate filaments

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

552 residues, UniProt reviewed canonical sequence.

>Q494X3|ZNF404
     1  MARVPLTFSD VAIDFSQEEW EYLNSDQRDL YRDVMLENYT NLVSLDFNFT TESNKLSSEK
    61  RNYEVNAYHQ ETWKRNKTFN LMRFIFRTDP QYTIEFGRQQ RPKVGCFSQM IFKKHKSLPL
   121  HKRNNTREKS YECKEYKKGF RKYLHLTEHL RDHTGVIPYE CNECGKAFVV FQHFIRHRKI
   181  HTDLKPYECN GCEKAFRFYS QLIQHQIIHT GMKPYECKQC GKAFRRHSHL TEHQKIHVGL
   241  KPFECKECGE TFRLYRHMCL HQKIHHGVKP YKCKECGKAF GHRSSLYQHK KIHSGEKPYK
   301  CEQCEKAFVR SYLLVEHQRS HTGEKPHECM ECGKAFGKGS SLLKHKRIHS SEKLYDCKDC
   361  GKAFCRGSQL TQHQRIHTGE KPHECKECGK TFKLHSYLIQ HQIIHTDLKP YECKQCGKAF
   421  SRVGDLKTHQ SIHAGEKPYE CKECGKTFRL NSQLIYHQTI HTGLKPYVCK ECKKAFRSIS
   481  GLSQHKRIHT GEKPYECKEC DKAFNRSDRL TQHETIHTGV KPQKCKECGK AFSHCYQLSQ
   541  HQRFHHGERL LM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF404 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 17 nTPM

Expression across tissuesHPA

Tissue

retina: 17 nTPM
pituitary gland: 14 nTPM
spinal cord: 12 nTPM
midbrain: 9.9 nTPM
cerebellum: 9.4 nTPM
epididymis: 9.1 nTPM

Single-cell type

cardiomyocytes: 60 nCPM
astrocytes: 29 nCPM
bergmann glia: 27 nCPM
oligodendrocytes: 20 nCPM
pituicytes/fscs: 19 nCPM
adipocytes: 17 nCPM

Immune cell

NK-cell: 0.5 nTPM
eosinophil: 0.4 nTPM
memory CD4 T-cell: 0.3 nTPM
naive B-cell: 0.3 nTPM
naive CD4 T-cell: 0.3 nTPM
naive CD8 T-cell: 0.3 nTPM

Brain region

white matter: 15 nTPM
thalamus: 12 nTPM
basal ganglia: 11 nTPM
medulla oblongata: 11 nTPM
pons: 11 nTPM
spinal cord: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.18
gnomAD pLI: 0
gnomAD missense Z: 0.99
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of transcription by RNA polymerase II

Molecular functions

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF404 as an antibody target. Whether an autoantibody or antibody against ZNF404 could matter depends on whether native ZNF404 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF404 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF404 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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