ZNF354A

Protein identityUniProt · HPA

UniProt accession

O60765

Gene

ZNF354A

Ensembl

ENSG00000169131

Chromosome

5

Canonical length

605 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in cytosol; nucleolus; and nucleoplasm. Biomarker of in situ carcinoma and seminoma. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

605 residues, UniProt reviewed canonical sequence.

>O60765|ZNF354A
     1  MAAGQREARP QVSLTFEDVA VLFTRDEWRK LAPSQRNLYR DVMLENYRNL VSLGLPFTKP
    61  KVISLLQQGE DPWEVEKDGS GVSSLGSKSS HKTTKSTQTQ DSSFQGLILK RSNRNVPWDL
   121  KLEKPYIYEG RLEKKQDKKG SFQIVSATHK KIPTIERSHK NTELSQNFSP KSVLIRQQIL
   181  PREKTPPKCE IQGNSLKQNS QLLNQPKITA DKRYKCSLCE KTFINTSSLR KHEKNHSGEK
   241  LFKCKECSKA FSQSSALIQH QITHTGEKPY ICKECGKAFT LSTSLYKHLR THTVEKSYRC
   301  KECGKSFSRR SGLFIHQKIH AEENPCKYNP GRKASSCSTS LSGCQRIHSR KKSYLCNECG
   361  NTFKSSSSLR YHQRIHTGEK PFKCSECGRA FSQSASLIQH ERIHTGEKPY RCNECGKGFT
   421  SISRLNRHRI IHTGEKFYNC NECGKALSSH STLIIHERIH TGEKPCKCKV CGKAFRQSSA
   481  LIQHQRMHTG ERPYKCNECG KTFRCNSSLS NHQRIHTGEK PYRCEECGIS FGQSSALIQH
   541  RRIHTGEKPF KCNTCGKTFR QSSSRIAHQR IHTGEKPYEC NTCGKLFNHR SSLTNHYKIH
   601  IEEDP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF354A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

12 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.78

gnomAD pLI

0

gnomAD missense Z

1

DepMap mean gene effect

0.23

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of transcription by RNA polymerase II
• sensory perception of sound

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• zinc ion binding

Cellular components

• cytosol
• nucleolus
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Krueppel-associated box
• Zinc finger C2H2-type
• Krueppel-associated box domain superfamily
• Zinc finger C2H2 superfamily
• Zinc finger, C2H2 type
• KRAB box

KeywordsUniProt

• Activator
• DNA-binding
• Isopeptide bond
• Metal-binding
• Nucleus
• Repeat
• Transcription
• Transcription regulation
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of ZNF354A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TRIM28